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JoVE Journal Developmental Biology
Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

Article DOI: 10.3791/65090-v • 07:35 min • February 17th, 2023
February 17th, 2023 •
1,2, 1,2, 3, 1,2,4, 1,2,4
1Department of Internal Medicine - Nephrology, Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory, Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center

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Summary
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Here, we present a detailed protocol for the transplantation of kidney organoids in the celomic cavity of chicken embryos. This method induces vascularization and enhanced maturation of the organoids within 8 days and can be used to study these processes in an efficient manner.

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Efficient Vascularization Kidney Organoids Intracoelomic Transplantation Chicken Embryos In Vitro Immature Applicability Maturation Procedure Transplantation Analysis HIPSC-derived Kidney Organoids Fertilized Leghorn Eggs Window Eggshell Transparent Tape Hole 19-gauge Needle Five-milliliter Syringe Albumin Embryo Conceal Pencil Marked Upward Facing Side Circular Window Curved Dissecting Scissors
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