Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

Haoming Chen; Bing Li; Hangbing Cao; Yehong Zhao; Yuanjie Zou; Wenyang Wang; Min Mu; Xinrong Tao

doi:10.3791/65127

Journal

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Medicine

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Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

JoVE Journal
Medicine

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JoVE Journal Medicine

Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

710 Views

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06:12 min

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March 03, 2023

DOI:

10.3791/65127-v

DOI:

10.3791/65127-v

•

06:12 min

March 03, 2023

•

702 Views

Haoming Chen*^1,2,3,4, Bing Li*^1,2,3,4, Hangbing Cao^1,2,3,4, Yehong Zhao^1,2,3,4, Yuanjie Zou^1,2,3,4, Wenyang Wang^1,2,3,4, Min Mu^1,2,3,4, Xinrong Tao^1,2,3,4

¹Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education,Anhui University of Science and Technology ²Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes,Anhui University of Science and Technology ³Anhui Province Engineering Laboratory of Occupational Health and Safety ⁴School of Medicine, Department of Medical Frontier Experimental Center,Anhui University of Science and Technology

Summary

This study describes a mouse model to study the synergistic effect of nicotine on the progression of pulmonary fibrosis in experimental silicosis mice. The dual-exposure mouse model simulates the pathological progression in the lung after simultaneous exposure to nicotine and silica. The methods described are simple and highly reproducible.