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Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

DOI:
10.3791/65134-v

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09:03 min

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May 19, 2023

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, ,
1Graduate School of Medicine,Yamaguchi University, 2Theodor Kocher Institute,University of Bern, 3Department of Neurotherapeutics,Yamaguchi University of Medicine

Chapters

  • 00:04Introduction
  • 01:33Magnetic Activated Cell Sorting (MACS) to Purify CD31+ Endothelial Progenitor Cells (EPCs)
  • 05:27Selective Passage to Purify EECM‐BMEC‐Like Cells and SMLCs
  • 07:07Results: Immunofluorescence Staining, Flow Cytometry Analysis, and Immune Cell Adhesion
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.

Tags

Keywords: Human-induced Pluripotent Stem CellsBrain Microvascular Endothelial CellsBlood-brain BarrierImmune Cell MigrationMultiple SclerosisStrokeNeuroinfectionEndothelial Progenitor CellsDissociationCell CultureCell CountingCentrifugationFlow Cytometry

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