Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Embedded 3D Printing within SHAPE composites overcomes traditional biofabrication limitations to enable accurate patterning of mechanically-sensitive tissues inside hydrogels that resemble the native extracellular environment. SHAPE toolbox relies on modular biomaterial design, allowing easy changes in printing support formulation. It also leverages low cost materials and accessible equipment for simple adaptation by other research groups.

This approach can be applied to create spatially-defined human neural tissue models for examining neuronal development and communication in neurodegenerative disorders such as Parkinson's. Begin by preparing calcium carbonate solution in ultrapure water at two milligrams per milliliter concentration. Mix it with the alginate solution at an equal ratio and magnetically stir it at 650 RPM for one hour at room temperature.