/

/

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

3,137 Views

•

10:50 min

•

April 07, 2023

DOI:

10.3791/65172-v

DOI:

10.3791/65172-v

•

10:50 min

April 07, 2023

•

3109 Views

Aymé Oliva Cárdenas^1,2, Blanca C. Zamora-Rodríguez², Kevin A. Batalla-García², Alejandro Ávalos-Rodríguez³, Alejandra Contreras-Ramos⁴, Clara Ortega-Camarillo²

¹Doctorado en Ciencias Biológicas y de la Salud,Universidad Autónoma Metropolitana ²Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI,Instituto Mexicano de Seguro Social ³Department of Agricultural and Animal Production, Division of Biological and Health Science,Universidad Autónoma Metropolitana-Xochimilco ⁴Molecular Biology Research Laboratory, Center for Congenital Malformations,Children Hospital of Mexico Federico Gomez (HIMFG)

Summary

This protocol describes a methodology for isolating and identifying adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats.

Explore More Videos

Mesenchymal Stem Cells Sprague Dawley Rats Cellular Communication Paracrine Function Pancreatic Regeneration Diabetes Therapies Allogenic Transplants Autologous Stem Cells Secretome Components Microarrays Next-generation Sequencing Oxidative Stress Stromal Vascular Fraction (SVF)Primary Cultures Fibroblast-like Morphology Flow Cytometry (FC)Immunofluorescence (IF)

Related Videos

Read Article