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Journal
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Biology
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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
小鼠肠隐窝和单个干细胞的类器官的3D培养用于类器官研究
JoVE Journal
Biology
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JoVE Journal
Biology
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Please note that all translations are automatically generated.
Click here for the English version.
小鼠肠隐窝和单个干细胞的类器官的3D培养用于类器官研究
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/65219-v
•
02:35 min
•
April 07, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Keywords: 3D Organoids
Intestinal Crypts
Intestinal Stem Cells
Homeostasis
Epithelial Growth
Differentiation
EDTA
Mechanical Dissociation
Signaling
Intestinal Epithelium
Paneth Cells
Enteroendocrine Cells
Enterocytes
Goblet Cells
Tuft Cells
Leucine-rich Repeats Containing G Protein-coupled Receptor 5
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