A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

Qing Liu; Lili Zhang; Rongxiang Fang; Yan Huo

doi:10.3791/65250

Journal

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Biology

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A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

JoVE Journal
Biology

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JoVE Journal Biology

A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

1,516 Views

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03:39 min

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April 28, 2023

DOI:

10.3791/65250-v

DOI:

10.3791/65250-v

•

03:39 min

April 28, 2023

•

29 Views

Qing Liu^1,3, Lili Zhang^1,2, Rongxiang Fang^1,2,3, Yan Huo^1,2

¹State Key Laboratory of Plant Genomics, Institute of Microbiology,Chinese Academy of Sciences, ²CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences, ³University of Chinese Academy of Sciences

Transcript

Our research focuses on arboviruses, insects, and plant interactions. Obtaining hemolymph samples from small arthropods presents a technical challenge for research on insect-borne diseases. This protocol shows a precise and quantifiable technique to counter this issue.

Recent research developments in the transmission of arboviruses have discovered that viruses can manipulate several important vector-factors to break through the host barrier. This protocol is a simple, cost-effective and valuable technique that allows for precise quantification of the hemolymph. This method also offers valuable technology for starting the interactions between viruses and vectors.

Our upcoming research will focus on understanding the molecular mechanisms of virus transmission by vector-insects while exploring and enhancing new operational techniques.