An improved method to measure pollen hydration profiles in Arabidopsis thaliana is described here. The new method offers higher resolution, is noninvasive, and is highly reproducible. The protocol represents a new tool for a finer dissection of the processes that regulate the early stages of pollination.
Lau, Y., Wang, L., Yang, M., Doughty, J. A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana. J. Vis. Exp. (196), e65280, doi:10.3791/65280 (2023).