Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

Luca Menozzi; &#193;ngela del &#193;guila; Tri Vu; Chenshuo Ma; Wei Yang; Junjie Yao

doi:10.3791/65319

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Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

1,210 Views

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06:45 min

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June 02, 2023

DOI:

10.3791/65319-v

DOI:

10.3791/65319-v

•

06:45 min

June 02, 2023

•

3 Views

Luca Menozzi*¹, Ángela del Águila*², Tri Vu¹, Chenshuo Ma¹, Wei Yang², Junjie Yao¹

¹Department of Biomedical Engineering,Duke University, ²Multidisciplinary Brain Protection Program, Department of Anesthesiology,Duke University School of Medicine

Transcript

There are a few experimental challenges. For example, the presence of hair in the scalp can cause artifact in the acquired images. The age of the animal also plays a key role since the thickness of the skull is currently a limitation for the detection of deeper signals.

Many purely optical imaging techniques require an invasive procedure and can only image about one millimeter deep. With PAUSAT, we combine the advantages of both ultrasound and optical imaging. We can get both structural and functional information surrounding ischemic stroke in a completely safe and non-invasive way.

Being able to evaluate the evolution of an injury with or without treatment from the same animal at different time points allows us to collect very valuable information. And also to reduce the number of animals required for different studies. We are constantly working on ways to improve the imaging speed, depth, resolution and contrast for both photoacoustic and ultrasound imaging.

With better imaging systems, we can answer more fundamental biological questions and evaluate new experimental treatments.

Summary

This work demonstrates the use of a multimodal ultrasound-based imaging platform for noninvasive imaging of ischemic stroke. This system allows for the quantification of blood oxygenation through photoacoustic imaging and impaired perfusion in the brain through acoustic angiography.