Free-Hand Intracerebroventricular Injections in Mice

Richard B. McCosh; Lauren A. Young

doi:10.3791/65324

Journal

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Neuroscience

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Free-Hand Intracerebroventricular Injections in Mice

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Free-Hand Intracerebroventricular Injections in Mice

9,954 Views

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05:29 min

•

January 12, 2024

DOI:

10.3791/65324-v

DOI:

10.3791/65324-v

•

05:29 min

January 12, 2024

•

126 Views

Richard B. McCosh¹, Lauren A. Young¹

¹Department of Biomedical Sciences,Colorado State University

Transcript

We are interested in the neural control of reproductive function, particularly how the brain regulates the release of hormones that control the gonads, both in healthy conditions and in response to stress. The main advantage of this technique is that it is relatively simple and rapid. Delivering drugs into the lateral ventricle is a great way to cast a wide net in testing a signaling pathway to support more detailed follow-up experiments.

There’s still much to be learned about the neuroendocrine control of reproduction, including the molecular mechanisms for luteinizing hormone pulse generation, mechanisms for modulating pulse frequency during stress, and generation of the preovulatory LH surge. In the future, we are particularly interested in integrating disparate findings into models.

Summary

Here, a simple and rapid approach for performing intracerebroventricular injections in mice using a free-hand approach (that is, without a stereotaxic device) is described.