Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development

Bortecine Sevgin; Merve Nur Coban; Faruk Karatas; Kerem Pekkan

doi:10.3791/65330

Journal

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Developmental Biology

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Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development

1,091 Views

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04:37 min

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June 16, 2023

DOI:

10.3791/65330-v

DOI:

10.3791/65330-v

•

04:37 min

June 16, 2023

•

1044 Views

Bortecine Sevgin*¹, Merve Nur Coban*¹, Faruk Karatas², Kerem Pekkan^1,2

¹Department of Biomedical Sciences and Engineering,Koc University ²Department of Mechanical Engineering,Koc University

Summary

Here, we present a detailed visual protocol for executing the left atrial ligation (LAL) model in the avian embryo. The LAL model alters the intracardiac flow, which changes wall shear stress loading, mimicking hypoplastic left heart syndrome. An approach to overcome the challenges of this difficult microsurgery model is presented.