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Journal
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Biology
/
Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche
/
小鼠骨骼肌的普通散装培养物，以概括生态位和干细胞静止
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Biology
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JoVE Journal
Biology
Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence
Please note that all translations are automatically generated.
Click here for the English version.
小鼠骨骼肌的普通散装培养物，以概括生态位和干细胞静止
Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche
DOI:
10.3791/65433-v
•
01:45 min
•
June 02, 2023
•
Louai Zaidan*
1
,
Perla Geara*
1
,
Matthew J. Borok*
1
,
Léo Machado
,
Despoina Mademtzoglou
,
Philippos Mourikis
,
Frederic Relaix
2,3,4
1
Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France
,
2
Ecole nationale vétérinaire d’Alfort, IMRB, F-94700 Maisons-Alfort, France
,
3
EFS, IMRB, F-94010 Creteil, France
,
4
AP-HP, Hopital Mondor, Service d’histologie, F-94010 Creteil, France
Tags
Keywords: Skeletal Muscle
Satellite Cells
Niche
Quiescence
Single-cell RNA Sequencing
FACS
In Vitro Culture
Myofibers
Stem Cells
Endothelial Cells
Fibro-adipogenic Progenitors
Regeneration
Homeostasis
Disease
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