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July 28, 2023
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Described here is a simple workflow to differentiate endothelial cells from human pluripotent stem cells followed by a detailed protocol for their mechanical stimulation. This allows for the study of the developmental mechanobiology of endothelial cells. This approach is compatible with downstream assays of live cells collected from the culture chip after mechanical stimulation.
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Cite this Article
Ventura, T., Egan, E. J., Romanò, N., Fidanza, A. In Vitro Model of Fetal Human Vessel On-chip to Study Developmental Mechanobiology. J. Vis. Exp. (197), e65492, doi:10.3791/65492 (2023).
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