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Journal
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Biology
/
Crude Vector Production and Efficient DNA Delivery in Cell Cultures
/
使用未纯化的重组腺相关病毒载体在培养细胞中进行转基因表达
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
Please note that all translations are automatically generated.
Click here for the English version.
使用未纯化的重组腺相关病毒载体在培养细胞中进行转基因表达
Crude Vector Production and Efficient DNA Delivery in Cell Cultures
DOI:
10.3791/65572-v
•
01:49 min
•
October 20, 2023
•
Brian Benyamini
,
Meagan N. Esbin
2
,
Oscar Whitney
,
Nike Walther
,
Anna C. Maurer
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
,
2
Biophysics Graduate Group
,
University of California, Berkeley
Tags
Keywords: Adeno-associated Viral Vectors
Gene Therapy
Transgene Expression
Cell Culture
Transduction
Gene Delivery
Recombinant AAV
Viral Capsid
DNA Processing
Cellular Machinery
Vectrology
Production Efficiency
Transduction Efficiency
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