Using Vertically Aligned Carbon Nanofiber Arrays on Rigid or Flexible Substrates for Delivery of Biomolecules and Dyes to Plants

Jessica M. Morgan; Joanna Jelenska; Dale K. Hensley; Pengju Li; Bernadeta R. Srijanto; Scott T. Retterer; Robert F. Standaert; Jennifer L. Morrell-Falvey; Jean T. Greenberg

doi:10.3791/65602

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Using Vertically Aligned Carbon Nanofiber Arrays on Rigid or Flexible Substrates for Delivery of Biomolecules and Dyes to Plants

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Using Vertically Aligned Carbon Nanofiber Arrays on Rigid or Flexible Substrates for Delivery of Biomolecules and Dyes to Plants

1,333 Views

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05:32 min

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July 21, 2023

DOI:

10.3791/65602-v

DOI:

10.3791/65602-v

•

05:32 min

July 21, 2023

•

17 Views

Jessica M. Morgan¹, Joanna Jelenska², Dale K. Hensley³, Pengju Li⁴, Bernadeta R. Srijanto³, Scott T. Retterer^3,5, Robert F. Standaert⁶, Jennifer L. Morrell-Falvey⁵, Jean T. Greenberg²

¹Biophysical Sciences,The University of Chicago, ²Molecular Genetics and Cell Biology,The University of Chicago, ³Center for Nanophase Materials Sciences,Oak Ridge National Laboratory, ⁴Pritzker School of Molecular Engineering,The University of Chicago, ⁵Biosciences Division,Oak Ridge National Laboratory, ⁶Department of Chemistry,East Tennessee State University

Transcript

We are trying to demonstrate a general method for delivery of dye or DNA into plants using carbon nanofiber arrays. Many plants are recalcitrant to traditional transformation methods. Our method can solve this problem using carbon nanofiber arrays on flexible rigid substrates.

Our technique is fast and user-friendly. It can be applied to virtually any plant species or plant organ. It can also be used to deliver other reagents such as dyes and proteins.

Summary

Here we describe methods for microfabricating vertically aligned carbon nanofibers (VACNFs), transferring VACNFs to flexible substrates, and applying VACNFs on both rigid and flexible substrates to plants for biomolecule and dye delivery.