Multiplex Immunohistochemistry Staining for Paraffin-embedded Lung Cancer Tissue

Our research focused on optimizing the mIHC protocol on paraffin section obtained from clinic sources. We aimed to effectively address autofluorescence and autofluorescence channel crosstalk issues in lung tissue. The current experimental challenges we are facing involve addressing the issues of ultrafluorescence and the channel crosstalk in lung tissue, thereby achieving successful what color fluorescence localization they need for labeled target cells and proteins.

Our highly efficient multiple antigen insight leveling method, which requires less than three hours per antibody, seamlessly integrates a multi-spectro emergent system and a specialized pathology software. This optimized mIHC protocol is easily adaptable for use in other research laboratories. To begin, obtain previously prepared formula and fixed and paraffin-embedded lung cancer tissue sample slide.