Name: Microfluidic Applications for Disposable Diagnostics (Video) | JoVE Journal
Uploaded: 2008-02-03T23:55:00.000+00:00
Duration: 10 min 21 s
Description: Learn thermoplastic microfluidic fabrication for disposable diagnostics using integrated sample preparation and nucleic acid extraction. This method enables rapid, field-deployable molecular detection in resource-limited settings.

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microfluidic applications for disposable diagnostics

Microfluidic Applications for Disposable Diagnostics

In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new diagnostics.

In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new diagnostics.

In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new ...

My name is Catherine CloudBridge and I'm a professor in manufacturing, engineering and biomedical engineering at Boston University. And I'm the director of the Biomedical Micro Devices and Microenvironments Laboratory. We work primarily on making disposable diagnostics for applications in resource limited settings.So we use microfluidics in order to make those devices. We're interested in actually two fields of study. Primarily we're interested in looking at how biomolecules and cells interact with plastics polymer surfaces.That's our major umbrella. That work is embodied in microfluidic applications for disposable diagnostics. So in the laboratory, what we make are microfluidic devices.In thermoplastic materials we don't use, typically use PDMS, which is the rubbery material that most people use to make microfluidics in the laboratory. We make our devices out of thermoplastics. We're interested in using those thermoplastic devices to do molecular biology at the microscale in such a way that that molecular biology could be done in the field far away from a centralized laboratory.How that works out in, in the lab is we we're making little plastic cards and inside of those little pla

microfluidic-applications-for-disposable-diagnostics

Research

JoVE Journal

Biology

We demonstrate protocols for manufacturing and automating elastomeric polydimethylsiloxane (PDMS)-based microvalve arrays that need no extra energy to close and feature photolithographically defined precise volumes.   A parallel subnanoliter-volume mixer and an integrated microfluidic perfusion system are presented.

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Development of New Therapeutic Applications Using Microfluidics

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

Fluorescence detection methods for microfluidic droplet platforms

An integrated microfluidic thermoplastic chip has been developed for use as a molecular diagnostic. The chip performs nucleic acid extraction, reverse transcriptase, and PCR. Methods for fabricating and running the chip are described.

Microfluidic Chip Fabrication and Method to Detect Influenza

In this article we present a microfluidic chip for single cell analysis. It allows the quantification of intracellular proteins, enzymes, cofactors, and second messengers by means of fluorescent assays or...

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

In this article we describe different techniques for microfluidic rapid prototyping platforms. The proposed techniques are based on ultraviolet (UV) sensitive and temperature curing epoxies, polydimethylsiloxane (PDMS) based tubing, wire-bonding, and anisotropic adhesive films. The assembling procedures presented are developed for both one-time use devices as well as reusable microfluidic systems.

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

We demonstrate a microfluidic platform with an integrated surface electrode network that combines resistive pulse sensing (RPS) with code division multiple access (CDMA), to multiplex detection and sizing of particles in multiple microfluidic channels.

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

A microfluidic biosensor platform was designed and fabricated using low-cost dry film photoresist technology for the rapid and sensitive quantification of various analytes. This single-use system allows for the electrochemical readout of on-chip-immobilized enzyme-linked assays by means of the stop-flow technique.

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Electrowetting-based digital microfluidic is a technique that utilizes a voltage-driven change in the apparent contact angle of a microliter-volume droplet to facilitate its manipulation. Combining this with functionalized magnetic beads enables the integration of multiple laboratory unit operations for sample preparation and identification of pathogens using Enzyme-linked Immunosorbent Assay (ELISA).

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Microfluidics is a powerful tool for the development of diagnostic tests. However, expensive equipment and materials, as well as laborious fabrication and handling techniques, are often required. Here, we detail the fabrication protocol of an acrylic microfluidic device for magnetic micro- and nanoparticle-based immunoassays in a low-cost and simple-to-use setting.

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Learn thermoplastic microfluidic fabrication for disposable diagnostics using integrated sample preparation and nucleic acid extraction. This method enables rapid, field-deployable molecular detection in resource-limited settings.

Microfluidic Applications for Disposable Diagnostics

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