In Vitro Culturing Technique for Studying Cellular Dynamics in Zebrafish Scales

Our research explores the effects of environmental disturbances of bone tissue. We are specifically interested in understanding the effects of simulated microgravity and vibration on adult and developing bone. We use zebrafish as a model organism of choice because it offers many advantages for biological research.

Our protocol offers advantages over previous protocols because it limits the need for a carbon dioxide incubator, and it uses standard laboratory equipment. Our lab is trying to unravel the communication dynamics between different types of bone cells when they are subjected to an environmental disturbance such as gravity or vibration. In the future, we would like to be able to mitigate the negative effects of space travel on bone tissue.