Development of a Bio-Hybrid Mosquito Stinger-Based Atomic Force Microscopy Probe

Nicolas Juncos Ljubich; Justin Puma; Zi Xin Zhang; Jianyu Li; Changhong Cao

doi:10.3791/66675

Journal

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Engineering

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Development of a Bio-Hybrid Mosquito Stinger-Based Atomic Force Microscopy Probe

JoVE Journal
Engineering

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JoVE Journal Engineering

Development of a Bio-Hybrid Mosquito Stinger-Based Atomic Force Microscopy Probe

710 Views

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04:51 min

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April 26, 2024

DOI:

10.3791/66675-v

DOI:

10.3791/66675-v

•

04:51 min

April 26, 2024

•

10 Views

Nicolas Juncos Ljubich¹, Justin Puma¹, Zi Xin Zhang¹, Jianyu Li¹, Changhong Cao¹

¹Department of Mechanical Engineering,McGill University

Transcript

We’re looking to develop an AFM cantilever to potentially quantitatively study the biting mechanisms of the mosquito. Our protocol offers a new method for creating accurate, controllable, and reliable AFM probes in order to study animal biting or stinging processes. Our protocol aims at accurately simulating the animal biting process, causing current methodologies to either be uncontrollable, unreproducible in a reliable manner, or in certain cases completely inaccurate.

The impact of our work is twofold. The first, we create a protocol that enables other researchers to fabricate their own custom bio-hybrid AFM cantilevers. Secondly, our work paves the way for creating other protocols that merge testing systems with biological material for creating more realistic testing scenarios.

Summary

Quantitative and controlled investigations into insect biting behaviors are crucial for devising effective strategies to combat vector-borne diseases. In this context, a method for fabricating a bio-hybrid atomic force microscopy (AFM) probe is introduced.