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Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
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Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Article DOI: 10.3791/674-v • 12:55 min • February 11th, 2008
February 11th, 2008 •
1, 2,3, 1, 4, 2, 4
1Biomedical Engineering Department, Cornell University, 2Neurosurgical Laboratory for Translational Stem Cell Research, Weill Cornell Brain Tumor Center, Weill Cornell Medical College of Cornell University, 3Cell Morphology Department, Instituto de Investigacion Principe Felipe, 4Department of Chemical and Biomolecular Engineering, Cornell University

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Summary
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We demonstrate that the over expression of epidermal growth factor receptors (EGFR) enhances the motility of neural stem cells(NSCs) using a novel agarose gel based microfluidic device. This technology can be readily adaptable to other mammalian cell systems where cell sources are scarce, such as human neural stem cells, and the turn around time is critical.

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Neural Stem Cells Motility Agarose Gel-based Microfluidic Device Microfluidic Technology Cell-based Assays Microenvironment Conventional Microfabrication Techniques Materials Agarose Gel Cell Migration Parallel Microfluidic Channels Peristaltic Pump Observation Chemical Environment Optical Monitoring Experimental Results
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