Paired Cisterna Magna Nanoinjection and Laser Speckle Contrast Imaging Assay to Study Cerebral Blood Flow Regulation In Vivo

To study cerebral blood flow regulation in vivo, traditional pharmacological methods are invasive, often damaging the skull, dura, and leptomeninges. In this work, we developed a minimally invasive technique that combines nanoinjector-guided intracisternal magna (ICM) injection with laser speckle contrast imaging, preserving the integrity of the skull and leptomeninges.