Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

My group is interested in the mechanisms that regulate gene expression in neural progenitor cells of the developing neocortex. And particularly, we focus on epigenetic mechanisms. These are important for the potential of neural progenitor cells to proliferate and differentiate, and therefore for proper brain development and function.

Cortical organoids are exciting new models that allow us to study the development of the human brain. They also allow us to model the development of other primate species. In this protocol, we describe the electroporation of human cortical organoids to study gene function.