Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Xuepu Jin; Binghuang Zhang; Yu Sun; Yahan Duan; Junchen Lu; Jiannan Liu; Jiahuai Han; Yingying Zhang

doi:10.3791/67616

Journal

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Genetics

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Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

JoVE Journal
Genetics

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JoVE Journal Genetics

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

518 Views

•

09:38 min

•

January 24, 2025

DOI:

10.3791/67616-v

DOI:

10.3791/67616-v

•

09:38 min

January 24, 2025

•

510 Views

Xuepu Jin*¹, Binghuang Zhang*¹, Yu Sun*¹, Yahan Duan¹, Junchen Lu¹, Jiannan Liu², Jiahuai Han^1,3,4, Yingying Zhang¹

¹State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences,Xiamen University ²Technology Center,Shanxi Xinghuacun Fenjiu Distillery Co., Ltd.³Research Unit of Cellular Stress of CAMS, Cancer Research Center of Xiamen University, Xiang’an Hospital of Xiamen University, School of Medicine,Xiamen University ⁴Laboratory Animal Center,Xiamen University

Summary

Here, we present a protocol to label proteins with a fluorescence protein tag in zebrafish larvae, a newly developed and modified in vivo system especially useful for visualizing low-abundance proteins in zebrafish.

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Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

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Summary

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