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JoVE Journal
Biology

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A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window
 

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

Article DOI: 10.3791/678 06:32 min
February 25th, 2008

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Summary February 25th, 2008

Cortical blood flow dynamics can be studied in vivo by imaging fluorescent dextran dyes injected into the tail vein of rodents with 2-photon microscopy. This video shows how to image blood flow dynamics in neocortex of mice through a glass-covered cranial window preparation.

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