Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo

Our study provides simplified in vitro and in vivo models to understand the impact of human immunosuppressive macrophages on CA 19 anti-tumor activity in the context of mantle cell lymphoma. The most commonly used model to study the human macrophages and monocytes in vivo is to engraft hematopoietic stem cells into eradicated immunodeficient mice. Engrafting immunodeficient mice with human hematopoietic stem cells to study human myeloid cells in mice can be time-consuming and expensive.

The engraftment efficiency can be limited as well. We establish more simplified in vitro and in vivo models to study interactions between human macrophages, CAR T, and tumor cells. It can be used to test other macrophage-targeted immunotherapies as well.