Application of 32P Radioactive Patch in Keloid Treatment

Summary

This protocol describes in detail the specific process and its efficacy for patients suffering from keloids who are treated with the ³²P Radioactive Patch. It covers medical equipment, lesion sampling, dressing device production, application methods, and post-operative care.

Abstract

Keloid, as a benign fibroblastic disease of the skin, is the result of excessive proliferation of fibroblasts and abnormal deposition of collagen during the process of skin trauma repair. Clinically, this disorder presents as a fibrous proliferation that protrudes beyond the surface of the skin beyond the margins of the original injury, affecting both function and aesthetics. In clinical treatment, the ³²P radioactive patch is a well-established and effective treatment based on the biological effect of β-rays, which can penetrate keloid tissue, induce apoptosis of fibroblasts in the keloid, impede cell proliferation, and inhibit excessive collagen synthesis and deposition, and eventually achieve the desired therapeutic effect after a period of treatment. However, because radiation naturally decays over time and because keloids have variability in dose requirements and size from patient to patient, ³²P radioactive patches usually need to be custom dispensed according to the patient's specific situation. This article will provide a detailed overview of the manufacturing process for ³²P radioactive patch radiation applicators, methods of calculating radiopharmaceutical doses, as well as specific procedures and considerations for clinical application.

Introduction

Keloids are benign skin lesions that can significantly impact appearance and are often associated with pain, burning sensations, itching, and, in some cases, impaired limb function, leading to considerable detriment to quality of life^1,2. During the skin wound healing process, keloids grow excessively due to the interplay of various factors, including molecular, cellular, physiological, biochemical, and physical elements. This interaction disrupts the body's repair mechanisms, resulting in the abnormal overgrowth of keloid tissues³. Keloids gradually protrude from the skin's sur....

Protocol

The study protocol was reviewed and approved by the Clinical Research Approval Committee of the First Affiliated Hospital, Zhejiang University School of Medicine [2025B] IIT Ethics Approval No.0225. Written informed consent was obtained from all subjects.

CAUTION: Radioactive compounds that emit ionizing radiation may present health risks to humans. Therefore, all procedures involving radioactive reagents are conducted with appropriate protective measures to minimize radiation exposure to operators and the surrounding environment. The use of radioactive compounds is strictly regulated at both institutional and national levels. The procedure....

Results

Material selection
For the preparation of phosphorus-32 (³²P) patches, various types of filter paper were sourced from reputable local suppliers. The insoluble matrix designed to retain radioactive substances must exhibit permeability, ductility, and liquid adsorption capabilities. The choice of filter paper type for impregnation with the appropriate medicinal solution significantly impacts the reliability of experimental outcomes. Owing to its balanced permeability and sufficient flexibil.......

Discussion

The use of ³²P radioactive patches for keloid treatment is among the most established and widely used methods in the clinical applications of nuclear medicine¹⁴. This approach involves applying the radioactive isotope patch close to the surface lesion, leveraging the biological effects of ionizing radiation to induce morphological and functional changes in the cells of the local lesion tissue to achieve the therapeutic purpose¹⁵.

The p.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
75% alcohol cotton balls	Cixi City Huabao Medical Supplies Industry & Trade Development Company	MQ/LJ-small	Cleansing and disinfecting the skin.
Filter Paper	Hangzhou Special Paper Industry Company	3#	The agent for adsorbing 32P solution.
Image J	National Institutes of Health(NIH)	Image processing software	https://imagej.net/ij/
Manual Pipettes	Eppendorf AG	3123000292   3123000233  3123000250	Accurate measurement and movement of medicinal liquids.
Medical disposable sterilization rubber gloves	Sri Trang Gloves(Thailand)Public Company Limited	F840	Protects hands from contamination.
Saline	China Otsuka Pharmaceutical Company	2C97B4	Dilute solution and configure drug.
Sodium Phospate[32P] Oral Solution	Atomic High Tech Company	H10960254	Provide 32P radioactive source.
Surface Contamination Monitor	IMI International Inc.	Inspector Alert V2	Surface contamination monitoring.
Transparent Film Dressing Frame Style	3M Company	1624W 9546HP	Closed filter paper, protecting the skin.