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DOI: 10.3791/69392-v
Elise Lupon1,2, Tanguy Perraudin1, Pierre Barbat1,2, Styliani Stergiadou3, Pharel Njessi1,2, Cassilia Dei2, Amina Oyuntogos1,2, Olivier Camuzard1, Didier F. Pisani2, Antoine Sicard2,4
1Department of Plastic and Reconstructive Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital,Université Côte d'Azur, 2Université Côte d'Azur, CNRS, LP2M, 3Anatomy Laboratory,University of Thessaly, 4Department of Nephrology, Dialysis and Kidney Transplantation,University Hospital of Nice
This protocol describes the surgical technique for harvesting a human perforator flap model based on the deep inferior epigastric artery pedicle, intended for experimental research.
Our study aims to improve preservation and survival of vascularized composite allografts, and free flaps using human experimental flaps models. Current research mainly relies on animal VCA and free flap models, often using rodent or large animal hand/limb osteomyocutaneous flaps. To begin, perform the preoperative dermal lipectomy marking with the patient and identify the midline from the pubic synthesis to the xiphoid process.
Draw a transverse line approximately seven centimeters above the vulvar commissure. Extend this line laterally about seven centimeters on each side, gently curving it upward to join just below the anterior superior iliac spines. Identify the perforators around the umbilicus using a handheld acoustic doppler probe with eight to 10 megahertz while the patient is in the supine position.
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