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Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
JoVE Journal
Biology
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JoVE Journal Biology
Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
DOI:

12:42 min

June 17, 2008

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Chapters

  • 00:03hESCs and Mitochondrial Complex Activity
  • 00:44Introduction
  • 01:41Mitochondrial Isolation
  • 04:08Solubilizing the Crude Mitochondrial Pellet
  • 05:42Gel and Sample Preparation and Electrophoresis Conditions
  • 09:47In-Gel Activity Assays
  • 12:08Conclusion

Summary

Automatic Translation

In this video, we will show you how the mitochondrial respiratory chain complexes of human embryonic stem cells can be analyzed using in gel activity assays.

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