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Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

JoVE Journal
Biology

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JoVE Journal Biology

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

DOI:

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13:02 min

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April 07, 2008

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G. Grant Welstead, Tobias Brambrink, Rudolf Jaenisch

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Chapters

00:10Cellular Reprogramming
03:30Introduction
04:32Transfecting HEK293T Cells and Purifying Lentivirus
06:08Infection of MEFs with Lentivirus
08:56Picking up the iPS Colonies
11:15Expansion of iPS Cells
12:32Conclusion

Summary

Automatic Translation

This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

Tags

IPS Cells MEFS Forced Expression Sox-2 Oct-4 C-Myc Klf4 Pluripotency Viral Transduction Reprogramming Strategy Doxycycline-inducible Lentiviral Vectors Induced Pluripotent Stem Cells Mouse Embryonic Fibroblasts Inducible Lentiviruses Retroviral Infection Gene Expression Analysis

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