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JoVE Journal Biology
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
 

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Article doi: 10.3791/772
May 9th, 2008 • Usage Statistics
1,2, 2
1Institute of Human Genetics and Northeast England Stem Cell Institute, Newcastle University, 2Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

Summary
Automatic Translation
May 9th, 2008

Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.

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