Hair Follicle Dissection
Hair Follicle Dissection - Related Videos
05:28
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
0 Views •
Cited by 1 •
2008
The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The...
07:00
Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
0 Views •
Cited by 19 •
2013
This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
0 Views •
Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
01:16
Accessory Structures of the Skin: Hair and Hair Follicles
0 Views •
2025
Hair and hair follicles are integral components of the integumentary system. Hair is a filamentous structure composed mainly of a protein called keratin. It is found on the surface of the skin throughout the body, except for areas such as the palms of the hands and soles of the feet. Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
03:18
Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicles
0 Views •
2025
This video demonstrates the combined recording of mechanically stimulated afferent output and nerve terminal labeling in the hair follicles of a mouse pinna. It outlines the steps involved in the mechanical stimulation of hair follicle nerve terminals before and after exposure to a fluorescent membrane dye, electrophysiology recording of the generated action potentials, and visualization of the nerve terminal labeling in the hair follicles.
FAQs
Separating epithelial regions, such as the outer root sheath and hair matrix, from the mesenchymal dermal papilla allows researchers to examine their interactions more precisely. These compartments provide distinct cellular material while retaining information about tissue organization. Comparing them helps clarify how epithelial and mesenchymal cells coordinate follicle development, cycling, and regeneration in biological studies.
Preserving tissue architecture maintains the spatial relationships among follicle regions that may be lost when components are separated too aggressively. This is especially important for microscopic analysis and for studying interactions between epithelial and mesenchymal cells. When viable material is needed, careful handling also supports subsequent cellular investigations rather than producing tissue suitable only for structural observation.
The downstream objective determines which features must be protected during separation. Microscopic studies prioritize recognizable tissue architecture, whereas molecular or cellular analyses require access to specific isolated regions and, when necessary, preservation of cell viability. Selecting the outer root sheath, dermal papilla, or hair matrix accordingly makes the resulting material more useful for the planned experiment.
A basic workflow uses fine instruments to separate the follicle into identifiable regions, including the outer root sheath, dermal papilla, and hair matrix. The operator then preserves either the tissue arrangement or cell viability according to the planned analysis. The isolated material can subsequently support microscopic, molecular, or cellular investigations, depending on how it was prepared.
Researchers use this technique when they need defined follicle regions as starting material for culture or transplantation. Isolation makes it possible to work with selected components rather than an undivided follicle, while appropriate preservation can maintain viability when required. These applications help investigate how follicular tissues behave outside their original arrangement or contribute to regenerative experiments.
In biology, dissected follicle regions provide material for examining stem cell behavior, pigmentation, wound repair, and skin disorders. They also support gene expression analyses that compare molecular activity among separated components. By linking cellular or molecular findings with follicle organization, the technique helps researchers study development, cycling, and regeneration in a more targeted way.