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JoVE Journal Biology
Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
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Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

Article DOI: 10.3791/820
November 5th, 2008 •
1, 1, 1
1Department of Pharmacology and Experimental Therapeutics, Tufts University

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November 5th, 2008

Primary dissociated midbrain dopamine cell cultures allow for the study of presynaptic characteristics of dopamine neurons. They can be used to monitor real-time dopamine release kinetics and protein/mRNA levels of regulators of dopamine exocytosis. Here, we show you how to generate these cultures from rodent neonates.

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