Journal
/
Biology
/
Double Whole Mount in situ Hybridization of Early Chick Embryos
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Double Whole Mount in situ Hybridization of Early Chick Embryos

Double Whole Mount in situ Hybridization of Early Chick Embryos

DOI:
10.3791/904-v

•

15:42 min

•

October 27, 2008

•

,
1Center for Environmental and Genetic Medicine,Institute of Biosciences and Technology – Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine,Texas A&M University (TAMU)

Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:16Fixing and Dehydrating the Embryos
  • 03:28Prehybridzation
  • 05:15Hybridizing Embryos with DIG- and FITC- Coupled Probes
  • 06:41DIG Antibody Incubation and Color Reaction
  • 09:40FITC Antibody Incubation and Color Reaction
  • 11:52Fixation and Processing
  • 13:34Results
  • 15:08Conclusion

Summary

Automatic Translation

Automatic Translation

This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.

Tags

Double Whole Mount In Situ HybridizationEarly Chick EmbryosEmbryonic DevelopmentGene ExpressionBrain DevelopmentNeural Tube FormationSomite FormationHeart Primordia Formation2-color Whole Mount In Situ HybridizationEmbryo DissectionParaformaldehyde FixationPrehybridizationProbe HybridizationDIG ProbeFITC ProbeOvernight HybridizationDIG Coupled AntibodyFITC Coupled AntibodyColor Reaction For DIG SubstrateColor Reaction With FITCPhotograph And SectioningTroubleshooting Guide

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/904?article_type=v

Method for Culture of Early Chick Embryos ex vivo (New Culture)
check_url/904?article_type=v

Method for Whole Mount Antibody Staining in Chick
check_url/904?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/904?article_type=v

Single Cell Transfection in Chick Embryos
check_url/904?article_type=v

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
check_url/904?article_type=v

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
check_url/904?article_type=v

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
check_url/904?article_type=v

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
check_url/904?article_type=v

RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs
check_url/904?article_type=v

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Read Article