Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

Hello, I'm IL OV to study the coordination of different lipid synthesis pathways to remember and biogenesis. It's useful to have an experimental system where membrane biogenesis of occur rapidly and induc. We found that phagocytosis of latex beats is particularly useful for such studies because it cells very quickly synthesize new membranes to replenish those that were lost as wrapping material early during the engulfment process.

In this video, my colleague Ong Zang will demonstrate procedures were used to study phagocytosis induced changes in gene expression using I Luciferase based report assay approach where, Hi, I'm X from the leverage of exon North in the Department of Molecular and the cellular biology at Harvard University. Today I will show you how to use a report gene assay to analyze the regulation of genes involved in membrane biogenesis after phagocytosis. So let's get us started.