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Journal / Biology / Optical Mapping of Langendorff-perfused Rat Hearts
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Optical Mapping of Langendorff-perfused Rat Hearts
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Optical Mapping of Langendorff-perfused Rat Hearts

Article DOI: 10.3791/1138-v • 11:48 min • August 11th, 2009
August 11th, 2009 •
1, 1,2, 1
1Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, 2Departments of Cardiac Surgery, Children's Hospital Boston and Harvard Medical School

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This article describes a high temporal and spatial resolution technique to optically image action potential movement on the surface of Langendorff-perfused rat hearts using a potentiometric dye (di-8-ANEPPS).

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Optical Mapping Langendorff-perfused Rat Hearts Voltage-sensitive Fluorescent Dyes Electrical Excitation Experimental Models Action Potentials Arrhythmias Spatiotemporal Resolutions Custom-built Equipment Electrophysiological Methods Complementary Metal-oxide-semiconductor (CMOS) Cameras Light-emitting Diodes (LEDs) Voltage-sensitive Dyes Optics Filters Cardiovascular Cell Biologists Microscopes Fluorescence Acquisition Technologies Photodiode Arrays Laser Scanning Systems Cooled Charged-coupled Device (CCD) Cameras
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