Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Fast and Sensitive Colloidal Coomassie G-250…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Journal (Biology)
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
Journal (Biology)
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
 
Click here for the English version

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels

Article DOI: 10.3791/1431-v • 10:57 min • August 3rd, 2009
August 3rd, 2009 •
1, 1
1Biological Medical Research Center (BMFZ), University Duesseldorf

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

This video shall popularize a colloidal Coomassie G-250 staining protocol according to Kang et al. for the detection of average 4 ng protein in gels. The staining is completed within 2 hours and without any effort. We routinely use Kang's protocol for analytical purposes in gel-based proteomics.

Tags

Coomassie Brilliant Blue G-250 Protein Staining SDS-PAGE Silver Staining Fluorescence Staining Mass Spectrometric Protein Identification Analytical Gel-based Proteomic Approaches Sensitivity Enhancement Low-abundant Proteins Colloidal Particles Methanol Ammonium Sulfate Phosphoric Acid Blue Silver Staining Kang Et Al. Aluminum Sulfate Ethanol
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter