Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / One-step Metabolomics: Carbohydrates, Organic and …
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
One-step Metabolomics
5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
Journal (Biology)
5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells
En Face Preparation of Mouse Blood Vessels
Journal (Biology)
En Face Preparation of Mouse Blood Vessels
 
Click here for the English version

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Article DOI: 10.3791/2014-v • 09:29 min • June 25th, 2010
June 25th, 2010 •
1
1Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

The urease method of sample preparation for GC/MS analysis of intermediary metabolites is presented by its inventor. The method allows one-step follow-up of newborn screening for inborn errors by tandem mass spectrometry by quantifying carbohydrates, organic and amino acids all in a single process.

Tags

One-step Metabolomics Carbohydrates Organic Acids Amino Acids Tandem Mass Spectrometry Acylcarnitines Organic Acidemias Amino Acid Quantification Enzymatic Testing Carbohydrate Disorders Urease Method Sample Preparation Gas Chromatography Dehydration Derivatization Trimethylsilylation Catalyst Triethylammonium Trifluoroacetate Automated Injection Chromatography Quantification Of Metabolites Semi-quantification Mass Spectra TMS Derivatized Biological Compounds
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter