Crystallization of Membrane Proteins in Lipidic Mesophases



The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals.

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Liu, W., Cherezov, V. Crystallization of Membrane Proteins in Lipidic Mesophases. J. Vis. Exp. (49), e2501, doi:10.3791/2501 (2011).


Membrane proteins perform critical functions in living cells related to signal transduction, transport and energy transformations, and, as such, are implicated in a multitude of malfunctions and diseases. However, a structural and functional understanding of membrane proteins is strongly lagging behind that of their soluble partners, mainly, due to difficulties associated with their solubilization and generation of diffraction quality crystals. Crystallization in lipidic mesophases (also known as in meso or LCP crystallization) is a promising technique which was successfully applied to obtain high resolution structures of microbial rhodopsins, photosynthetic proteins, outer membrane beta barrels and G protein-coupled receptors. In meso crystallization takes advantage of a native-like membrane environment and typically produces crystals with lower solvent content and better ordering as compared to traditional crystallization from detergent solutions. The method is not difficult, but requires an understanding of lipid phase behavior and practice in handling viscous mesophase materials. Here we demonstrate a simple and efficient way of making LCP and reconstituting a membrane protein in the lipid bilayer of LCP using a syringe mixer, followed by dispensing nanoliter portions of LCP into an assay or crystallization plate, conducting pre-crystallization assays and harvesting crystals from the LCP matrix. These protocols provide a basic guide for approaching in meso crystallization trials; however, as with any crystallization experiment, extensive screening and optimization are required, and a successful outcome is not necessarily guaranteed.


A typical outline of an in meso crystallization experiment is shown in Fig.11,2. Pre-crystallization LCP-FRAP assays are optional; however, they can significantly accelerate the process of searching for initial crystallization conditions, especially in the case of difficult membrane proteins3.

1. Protein Reconstitution in LCP

  1. Purify a membrane protein of interest in a detergent solution and concentrate the protein/detergent complexes to ~10 - 20 mg/mL, taking care not to over-concentrate the detergent1,4.
  2. Transfer ~25 mg of an LCP host lipid (typically monoolein) or a lipid mixture into a 1.5 mL plastic tube and incubate at 40 °C for few minutes until the lipid melts.
  3. Attach a syringe coupler to a 100 μL gas-tight syringe.
  4. Load the syringe with the molten lipid using an adjustable volume pipette. Record the volume of the lipid in the syringe.
  5. Load another 100 μL syringe with the protein solution at a protein solution-to-lipid ratio 2/3 v/v.
  6. Connect both syringes together through the syringe coupler.
  7. Push the syringe plungers alternately to move the lipid and protein through the inner needle of the coupler, back and forth, until the lipid mesophase becomes homogeneous. LCP forms spontaneously upon mechanical mixing, and the protein becomes reconstituted in the lipid bilayer of LCP. Formation of LCP can be verified by its transparent and gel-like consistency and by the absence of birefringency when viewed under a microscope equipped with cross-polarizers, or, if possible, by using small-angle X-ray diffraction1.

2. LCP-FRAP Pre-crystallization Assays

LCP-FRAP assays are designed to measure the diffusion properties of membrane proteins reconstituted in LCP at a variety of screening conditions3. The long-range diffusion of membrane proteins in LCP is essential for successful crystallization; however, the microstructure of LCP constrains diffusion of large proteins or oligomeric protein aggregates. A common reason for failure of an in meso crystallization experiment is a fast protein aggregation leading to a loss of diffusion. It has been shown that the aggregation behavior of a protein depends on the particular protein construct, the host lipid and the composition of the screening solution3.

  1. Label the protein with a fluorescent dye (Cy3 or similar) at a protein/dye ratio of ~100/1, remove the unreacted dye and concentrate the protein to ~ 1 mg/mL. Label either free amines or free thiols. When labeling free amines, use pH between 7 and 7.5 to predominately label the free N-terminus. Be aware that amino labeling can also label lipids co-purified with the protein2,3.
  2. Reconstitute the labeled protein in LCP as described in section 1).
  3. Set up assay plates as described in section 3) using LCP-FRAP screening solutions instead of crystallization screens2.
  4. Incubate the plates at 20 °C in the dark for at least 12 hours to achieve an equilibrium state.
  5. Place one of the plates on the LCP-FRAP station and focus on the first well using a 10x objective.
  6. Acquire 5 fluorescent images to capture the initial pre-bleached state.
  7. Trigger the laser. The laser power and number of pulses should be adjusted to bleach ~30 - 70% of the labeled protein in the middle of the bleached spot.
  8. Immediately after triggering the laser, start recording a fast post-bleaching sequence of ~200 images at the fastest possible rate.
  9. Follow with recording of a slow post-bleach sequence of ~50 images, selecting the delay between images as 1-20 s, depending on the diffusion rate of the protein.
  10. Integrate the intensity inside the bleach spot in all frames and correct it for bleaching and light intensity fluctuations during the acquisition by dividing the intensity inside the bleached spot by the averaged intensity of a reference spot outside of the laser bleached area.
  11. Normalize the corrected intensity to make the pre-bleached intensity equal to 1 and the initial bleached intensity equal to 0.
  12. Fit the curve of the normalized intensity vs. time, F(t), using the following equation5:
    F(t) = M x exp(-2T/t) x (I0(2T/t) + I1(2T/t)), (Eq.1)
    where M is the mobile fraction of diffusing molecules, T is the characteristic diffusion time, t is the real time of each recorded frame, I0 and I1 are the 0th and 1st order modified Bessel functions.
  13. Calculate the diffusion coefficient, D, as:
    D = R2/4T, (Eq.2)
    where R is the radius of the bleached spot.
  14. Move to the next well and repeat steps 2.5) - 2.13).
  15. Compare the mobile fractions and diffusion coefficients obtained for the different screening conditions. Design new crystallization screens based on the components that facilitated protein diffusion and excluding conditions for which protein diffusion was not observed. If the protein did not diffuse in any of the screened conditions, consider broadening the screening space or trying a new protein construct.

3. Setting Up LCP Crystallization Trials

  1. Reconstitute the protein in LCP as described in section 1).
  2. Transfer the protein-laden LCP into a 10 μL gas-tight syringe attached to a repetitive syringe dispenser.
  3. Attach a short removable needle (gauge 26, 10 mm length) to the 10 μL syringe.
  4. Dispense 200 nL boluses of LCP on the surface of four adjacent wells forming a 2x2 square.
  5. Overlay each of the LCP boluses with 1 μL of corresponding crystallization screen solution.
  6. Cap four loaded wells with an 18 mm square glass coverslip. Apply a gentle pressure on the coverslip to seal the wells.
  7. Repeat steps 3.4)-3.6) with the next set of 4 wells until the whole plate is filled.
  8. Incubate the plate at a constant temperature, periodically checking for crystal formation and growth.

4. Harvesting Crystals from LCP

  1. Place a plate with protein crystals under a stereo microscope with variable zoom, equipped with a linear rotating polarizer and analyzer.
  2. Focus on the well of interest using a low power zoom so that the whole well is placed within the field of view.
  3. Score the coverslip glass in four strokes making a square inside the well boundaries using a sharp corner of a ceramic capillary cutting stone.
  4. Press around the scored perimeter with strong sharp-point tweezers to propagate the scratches through the thickness of the coverslip glass.
  5. Punch two small holes at opposite corners of the scored square.
  6. Inject few μL of precipitant solution through one of the holes to reduce dehydration during the subsequent steps.
  7. Using an angled sharp needle probe break up the glass along one or two sides to free the cut-out square.
  8. Carefully lift up the glass square watching for the cubic phase bolus. If the bolus is stuck to the coverslip, then flip the glass square over and place on the bottom of the well.
  9. Add an extra few μL of precipitant solution, supplemented with a cryo-protectant, if necessary, on top of the exposed cubic phase bolus in the well.
  10. Increase magnification of the microscope and focus on a crystal.
  11. Adjust the angle between the polarizer and the analyzer to increase the contrast between the birefringent crystal and the background, while keeping enough light to see the harvesting loop.
  12. Select a MiTeGen MicroMount with a diameter matching the crystal size and then harvest the crystal directly from the LCP by scooping it into the MicroMount.
  13. Flash freeze the MicroMount with the harvested crystal in liquid nitrogen, and ship it to a synchrotron source beamline for X-ray data collection6.

5. Representative Results:

An engineered human beta 2 adrenergic G protein-coupled receptor (β2AR-T4L) was expressed in baculovirus infected sf9 insect cells and purified in dodecylmaltoside (DDM)/ cholesteryl hemisuccinate (CHS) detergent solution bound to a partial inverse agonist carazolol7. The protein was labeled with Cy3 NHS ester and used in LCP-FRAP pre-crystallization assays (Figure 2). Coarse grid screens based on several conditions selected from the results of LCP-FRAP assays produced initial crystal-like hits (Figure 3). Further optimization of precipitant conditions yielded diffraction quality crystals (Figure 4).

Figure 1
Figure 1. Flow-chart of a typical LCP crystallization experiment. Steps in the gray boxes are not described in the current protocols.

Figure 2
Figure 2. LCP-FRAP assay with β2AR-T4L/carazolol in monoolein based LCP. A) Results of an LCP-FRAP assay performed in an automatic high-throughput mode, in which each sample of a 96-well plate is bleached sequentially and fluorescence recovery is measured after a 30 min incubation. The obtained fluorescence recoveries, which represent the mobile fraction in each sample, are plotted for all 96 samples. The screening solutions contain 0.1 M Tris pH 8, 30 % v/v PEG 400 combined with 48 different salts at two different concentrations. B) Fluorescence recovery profiles for several representative conditions. Solid line curves represent fits by Eq. 1.The mobile fractions and the diffusion coefficients are determined using Eqs. 1 and 2. Fast recovery of less than 10% in the sample containing Na chloride is due to fluorescently labeled lipids co-purified with the protein.

Figure 3
Figure 3. Initial crystal hits of β2AR-T4L/carazolol obtained by a coarse grid screening around most promising conditions identified by LCP-FRAP, containing Na sulfate (panel A) and Na Formate (panel B). The protein is labeled with Cy3 NHS ester and the fluorescent images are taken using excitation at 543 nm and emission at 605 nm.

Figure 4
Figure 4. Optimized crystals of β2AR-T4L/carazolol. The images of crystals grown in the presence of Na sulfate (panels A and B) and K Formate (panels C and D) are taken in the brightfield mode (panels A and C) and using cross-polarizers (panels B and D).


The protocols provide a basic visual guidance for the main steps involved in performing in meso crystallization experiments. More in-depth details related to these protocols, emphasizing possible pitfalls, shortcomings or alternative routes are available elsewhere1,2. Optional LCP-FRAP assays can help at the earlier stages to select the most promising protein construct, LCP host lipid and lipid additives, as well as limit the range of possible precipitants and buffer conditions3. Once an initial crystallization hit is found, it should be optimized to obtain better quality crystals. Optimization of in meso crystallization conditions is essentially similar to optimizing conditions for soluble proteins with the addition of extra parameters associated with the composition of LCP1. Membrane protein crystals grown in lipidic mesophase are typically smaller in size than crystals obtained in detergent solution, but more ordered, thus, benefitting strongly from using microfocus beamlines available at modern synchrotron sources6.

Many of the procedures related to in meso crystallization, including setting up crystallization or assay plates, conducting LCP-FRAP assays and detecting crystals, have been semi- or fully automated1,2,8,9, allowing screening of a large range of conditions while consuming small amounts of protein and lipid. On the other hand, protein reconstitutions in LCP and crystal harvesting remain manual and more tedious operations and, thus, have a need for improvement.


No conflicts of interest declared.


This work was funded in parts by the NIH grants GM073197 and RR025336.


Name Company Catalog Number Comments
100 μL gas-tight syringe Hamilton Co 7656-01 2 syringes are required
10 μL gas-tight syringe Hamilton Co 7653-01
Syringe coupler Hamilton Co 7770-02 30902 The coupler can be made using available parts as described in refs. 10
Repetitive syringe dispenser Hamilton Co 83700 The repetitive syringe dispenser can be modified to reduce dispensing volume by ~3 times11
Short (0.375”) flat-tipped removable needle (point style 3, gauge 26) Hamilton Co 7804-03
96-well glass sandwich plate Marienfeld 08 900 03 For manual operations it is more convenient to assemble the glass sandwich plate using a standard microscope glass slides and a double sticky tape with punched holes1,2,12.
Glass cover slip Electron Microscopy Sciences 63787-01
monoolein Sigma-Aldrich M7765
Crystallization screens Hampton Research, Molecular Dimensions, Emerald Biosystems, Jena Bioscience Most of available commercial screens can be used for initial screening. Conditions that consistently disrupt LCP can be diluted 2x for better compatibility13.
Capillary cutting stone Hampton Research HR4-334
Fine point tweezers Ted Pella, Inc. 510
Angled sharp probe Ted Pella, Inc. 13650
MicroMounts MiTeGen M1-Lxx-xx Select MicroMount diameter to match the crystal size



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  8. Cherezov, V., Peddi, A., Muthusubramaniam, L., Zheng, Y. F., Caffrey, M. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr. D. 60, 1795-1807 (2004).
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  11. Cherezov, V., Caffrey, M. A simple and inexpensive nanoliter-volume dispenser for highly viscous materials used in membrane protein crystallization. J. Appl. Cryst. 38, 398-400 (2005).
  12. Cherezov, V., Caffrey, M. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. J. Appl. Cryst. 36, 1372-1377 (2003).
  13. Cherezov, V., Fersi, H., Caffrey, M. Crystallization screens: compatibility with the lipidic cubic phase for in meso crystallization of membrane proteins. Biophys J. 81, 225-242 (2001).



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    August 19, 2011 - 8:47 AM

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    February 6, 2012 - 5:22 AM

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