05:34Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.