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JoVE Journal Biology
Biofysische Testen om de mechanische eigenschappen van de interfase celkern Probe
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Biofysische Testen om de mechanische eigenschappen van de interfase celkern Probe: Basismateriaal Strain Toepassing en micronaaldjes Manipulatie

Article DOI: 10.3791/3087-v • 16:27 min • September 14th, 2011
September 14th, 2011 •
1, 1, 1,2
1Brigham and Women's Hospital / Harvard Medical School, Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering, Cornell University

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We presenteren twee onafhankelijke, microscoop-gebaseerde tools om de veroorzaakte nucleaire en cytoskelet vervormingen in enkele, levende hechtende cellen te meten in reactie op globale of lokale stam toepassing. Deze technieken worden gebruikt om de nucleaire stijfheid (dat wil zeggen, vervormbaarheid) te bepalen en om intracellulaire krachtoverbrenging tussen de kern en het cytoskelet sonde.

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Biofysica nucleaire envelop nucleaire stijfheid nucleo-cytoskelet koppeling Lamin nesprin cytoskelet biomechanica nucleaire vervorming krachtoverbrenging
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