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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Article DOI: 10.3791/3288-v • 10:37 min • February 7th, 2012
February 7th, 2012 •
1, 2, 2, 2, 1, 3, 3, 2
1Institute for Microstructural Sciences, National Research Council of Canada, 2Institute for Biological Sciences, National Research Council of Canada, 3Hotchkiss Brain Institute, University of Calgary

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Summary
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We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

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Culturing Electrophysiology Cells Patch-clamp Chips Sensitivity Ion Channels Gold Standard Disease Models Pharmaceutical Screens Glass Pipette Membrane Patch Electrode Ion-channel Activity Whole Cell Patch-clamp Chips Suspended Film Aperture Microfabricated Automated Systems High-throughput Screening Fluidic Delivery Suction Cell-to-probe Seals Whole Cell Mode Silicon Patch-clamp Chip Impedance Orifice Shape Action Potentials Snail Neurons Mammalian Neurons
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