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JoVE Journal Neuroscience
Testen DNA Damage in Hippocampus-Neuronen mit dem Comet Assay
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Testen DNA Damage in Hippocampus-Neuronen mit dem Comet Assay

Article DOI: 10.3791/50049-v • 10:00 min • December 19th, 2012
December 19th, 2012 •
1, 2, 1,3
1Department of Radiation Oncology, University of Alabama-Birmingham, 2Department of Radiation Oncology, The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

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Der Comet Assay ist ein effizientes Mittel zur Feststellung von Einzel-und Doppelstrang-Brüche, einschließlich Alkali-labile Websites und DNA-DNA/DNA-protein Cross-Links auf der DNA in allen Zellen, einschließlich Neuronen im Hippocampus. Die Methode nimmt den Vorteil dieser differentielle Migration von DNA in einem elektrischen Feld aufgrund von Unterschieden in Menge an DNA-Schäden.

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Neuroscience Ausgabe 70 Genetik Zellbiologie Medizin Cancer Biology Anatomie Physiologie DNA DNA-Schäden Doppelstrangbruch Einzelstrangbruch Reparatur Neuronen Comet Assay Zellkultur
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