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Immunofluorescenza combinato e FISH DNA 3D conservato nuclei in interfase di studiare i cambiamenti in 3D Organizzazione nucleare

Article DOI: 10.3791/50087-v • 13:55 min • February 3rd, 2013
February 3rd, 2013 •
1, 1,2,3,4, 1
1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

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Qui si descrive un protocollo per la rilevazione simultanea di modificazioni istoniche da sequenze di DNA di immunofluorescenza e FISH DNA seguita da microscopia e analisi 3D (3D immuno-DNA FISH).

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Genetica Biologia Molecolare Bioinformatica Cancer Biology Patologia Ingegneria Biomedica Immunologia Spazio intranucleari Nuclear Matrix Fluorescenza pesce pesce DNA 3D DNA immunofluorescenza immuno-FISH microscopia 3D organizzazione nucleare nuclei in interfase modificazioni della cromatina
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