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A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
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A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Article DOI: 10.3791/50722-v • 06:10 min • December 23rd, 2013
December 23rd, 2013 •
1, 1, 1
1Department of Biochemistry, University of Oxford

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Exonucleases play critical roles in ensuring genome stability. Loss of WRN exonuclease function results in premature aging. Studying substrates and other requirements of the nuclease in vitro can help elucidate its role in vivo. Here we demonstrate a rapid and reproducible fluorescence-based assay to measure its nuclease activity.

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Fluorescence-based Exonuclease Assay DmWRNexo WRN Exonuclease DNA Damage DNA Replication Genotoxins Recombinogenic Intermediates Hyper-recombinant Phenotype Helicase Activity Genome Stability Mechanisms Fluorescent Method Purified Recombinant DmWRNexo End-labeled Fluorescent Oligonucleotides Radioactive Assays Nuclease Polarity Processivity Substrate Preferences
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