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Developmental Biology

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JoVE Journal Developmental Biology
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Article DOI: 10.3791/52755-v • 08:00 min • May 14th, 2015
May 14th, 2015 •
1, 1, 1, 1, 2, 1, 1
1InvivoSciences, Inc., 2Gilson, Inc.

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The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.

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Scalable 96-well Plate IPSC Culture Robotic Liquid Handling System Pluripotent Stem Cells Regenerative Medicine Drug Discovery Safety Testing Cell-manufacturing Technology Pluripotency Maintenance Downstream Applications Large-scale Cell Production Manual Cell Culture Operator Variability Scale-up High-throughput Multi-channel Liquid Handling Robot Technician Involvement Experience Feeder-free Conditions Frozen Stock 96-well Plates
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