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JoVE Journal
Immunology and Infection

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Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
 

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Article DOI: 10.3791/54062-v 08:02 min June 27th, 2016
June 27th, 2016

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Summary

This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.

Tags

EAK 16-II/EAKIIH6 Peptide Thymic Epithelial Cells FACS Purification Self-assembling Hydrogel Three-dimensional Structure Thymus Bioengineering Cell Aggregation Biodegradable Injections C57 Black 6J Mouse Thymus Harvesting RPMI 1640
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