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Journal / Biochemistry / Detektering av den pH-beroende aktivitet för
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Biochemistry

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JoVE Journal Biochemistry
Detektering av den pH-beroende aktivitet för
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Journal (Biochemistry)
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
Journal (Biochemistry)
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
 
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Detektering av den pH-beroende aktivitet för

Article DOI: 10.3791/54527-v • 08:32 min • October 23rd, 2016
October 23rd, 2016 •
1, 1,2, 1,2, 1
1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

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Denna studie beskriver biofysiska, biokemiska och molekylära tekniker för att karakterisera chaperone aktiviteten hos Escherichia coli HdeB under sura pH-betingelser. Dessa metoder har tillämpats med framgång för andra syraskydds chaperoner såsom HdeA och kan modifieras för att fungera för andra chaperoner och stressförhållanden.

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Biokemi Molecular förkläde Acid Stress stress Protein Aggregation Protein Fälla bakterier Light Scattering Protein Återveck protein-proteininteraktion Protein kvalitetskontroll.
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