Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

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Summary

Previous work suggests that the nitrogen isotopic composition of atmospheric nitrogen oxides might distinguish the influence of different sources in the environment. We report on an automated, mobile, field-based method for the high collection efficiency of atmospheric NOx for N isotopic analysis at an hourly time resolution.

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Wojtal, P. K., Miller, D. J., O'Connor, M., Clark, S. C., Hastings, M. G. Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx. J. Vis. Exp. (118), e54962, doi:10.3791/54962 (2016).

Abstract

Nitrogen oxides (NOx = NO + NO2) are a family of atmospheric trace gases that have great impact on the environment. NOx concentrations directly influence the oxidizing capacity of the atmosphere through interactions with ozone and hydroxyl radicals. The main sink of NOx is the formation and deposition of nitric acid, a component of acid rain and a bioavailable nutrient. NOx is emitted from a mixture of natural and anthropogenic sources, which vary in space and time. The collocation of multiple sources and the short lifetime of NOx make it challenging to quantitatively constrain the influence of different emission sources and their impacts on the environment. Nitrogen isotopes of NOx have been suggested to vary amongst different sources, representing a potentially powerful tool to understand the sources and transport of NOx. However, previous methods of collecting atmospheric NOx integrate over long (week to month) time spans and are not validated for the efficient collection of NOx in relevant, diverse field conditions. We report on a new, highly efficient field-based system that collects atmospheric NOx for isotope analysis at a time resolution between 30 min and 2 hr. This method collects gaseous NOx in solution as nitrate with 100% efficiency under a variety of conditions. Protocols are presented for collecting air in urban settings under both stationary and mobile conditions. We detail the advantages and limitations of the method and demonstrate its application in the field. Data from several deployments are shown to 1) evaluate field-based collection efficiency by comparisons with in situ NOx concentration measurements, 2) test the stability of stored solutions before processing, 3) quantify in situ reproducibility in a variety of urban settings, and 4) demonstrate the range of N isotopes of NOx detected in ambient urban air and on heavily traveled roadways.

Introduction

Atmospheric nitrogen oxides (NOx = NO + NO2) are important species in the global reactive nitrogen cycle1,2. NOx in the atmosphere is highly reactive and directly contributes to the oxidizing capacity of the atmosphere through its interactions with ozone (O3) and hydroxyl radical (OH). NOx is removed from the atmosphere on the scale of hours to days in the lower troposphere via oxidation to nitric acid (HNO3) or nitrate (NO3-), both of which are highly soluble and can be dry deposited on surfaces in gaseous and particulate aerosol forms or wet deposited by precipitation (e.g., acid rain)2. NOx is emitted from a variety of sources, including fossil fuel combustion, biomass burning, microbial processes in soils, and lightning. Source apportionment is crucial for understanding the impacts of individual sources, but the variety of sources, their variability in space and time, and the relatively short lifetimes of NOx and HNO3 make concentration analyses alone an inadequate metric. Stable isotopes may be useful as a way to better track the spatial patterns and temporal trends of sources and the chemistry of NOx and NO3- in the environment and to add new constraints on atmospheric models3. To date, the isotopic signatures associated with different NOx sources remain highly uncertain, particularly because of large uncertainties associated with previous methods4.

Previous studies represent a number of different active and passive collection methods and yield large ranges in reported isotopic values, even for the same emission source. Fibiger et al. found that previously used methods often varied greatly in terms of their efficiency in capturing NOx, with changes in conditions greatly influencing field collection (e.g., temperature, humidity, flow rates, age of solution)4. The inefficient uptake of previous NO and NO2 capture methods could lead to fractionations. For example, higher rates of oxidation for 14N relative to 15N could yield low biases in δ15N-NOx that are not representative of atmospheric values. In addition to methodological issues4,17, a variety of different types of air sampling may also contribute to differences in the reported ranges for isotope values associated with the same source. For example, isotopic signatures associated with vehicle emissions of NOx have been suggested based on collections at near-road sites5, in traffic tunnels6, and directly from the tailpipes of vehicles7,8. Furthermore, previous methods have time resolutions of 24 h at best, and significant changes in ambient NOx concentrations are observed on hourly (or shorter) timescales9, potentially limiting the application of isotopic detection for different sources. Many of the NOx collection methods require very strong oxidizing solutions capable of oxidizing NOx, but also other collected reactive nitrogen species (e.g., ammonium), to nitrate over time, potentially contributing an isotopic measurement interference. Some previous methods are also limited to collecting NO2 in solution, which provides only a limited understanding of NOx isotopes, as it does not collect NO (the primary emission). Thus, there is a need to capture NOx from different emissions sources using a consistent, validated method to better constrain whether the variability in isotopes of NOx (and NO3-) in the environment can be used to directly track sources and chemistry.

This paper reports on a field-based NOx collection technique for isotopic analysis with the requisite time resolution, collection efficiency (100%), and reproducibility (≤1.5‰) for application in multiple field environments. The method, originally described by Fibiger et al.4, is further validated through the demonstration of its collection efficiency under changing NOx and meteorological conditions in the field, the test of solution stability and ammonia interferences, and the substantiation of its reproducibility in urban environments. Spatial and temporal differences in isotopic values are investigated using a single laboratory- and field-verified method that can capture NOx in solution at high efficiency. This paper demonstrates the application of the method for near-road, on-road, and ambient urban air collections at time resolutions of 30 to 120 min.

In brief, NOx (NO and NO2) is collected from the atmosphere in a highly oxidizing solution as NO3-. At the same time, ambient NOx, NO2, and CO2 concentrations and other relevant data, such as GPS location and time of collection, are recorded. After a sample is collected, the solution is processed in the laboratory, which involves reducing the solution to stop the reaction, then neutralizing the solution pH for subsequent NO3- concentration and isotopic analyses. The NO3- concentration is determined here by an automated spectrophotometric (i.e., colorimetric) process. The nitrogen isotopic composition is determined using the denitrifier method, which quantitatively converts the NO3- in solution to gaseous N2O that is subsequently measured on an isotope ratio mass spectrometer. Laboratory and field blanks are also collected and measured as part of the collections to ensure the sample integrity. Below is a detailed step-by-step protocol.

Protocol

1. Solution Preparation

  1. Before sampling, prepare the solutions, calibrate the NOx analyzer (either luminol or chemiluminescence), and check that the system is working properly and that new filters are installed.
  2. Make sampling solutions using 1 M potassium permanganate (KMnO4) stock solution and 10 M sodium hydroxide (NaOH)10, and then dilute the solution with ultrapure water to the correct volume.
    NOTE: Purchase premade solutions because they tend to contain lower "blank" NO3- concentrations than other forms.4
    1. Prepare 10 M NaOH.
      1. Weigh out 200 g of solid NaOH and pour it into a 500 ml volumetric flask. Add ultrapure water (18.2 MΩ·cm at 25 °C) to the meniscus line of the volumetric flask and allow the NaOH to dissolve.
    2. Because this process exudes heat, place the volumetric flask in a room-temperature (~22 °C) water bath and allow it to cool as it dissolves, usually taking 1-2 hr. Store 10 M NaOH in 500 ml amber plastic bottles for up to 1 month.
    3. Prepare a sampling solution of 0.25 M KMnO4 and 0.5 M NaOH in a 500 ml graduated cylinder (450 ml solution volume).
      1. Add 112.5 ml of 1 M KMnO4 and then fill with ultrapure water up to 405 ml.
      2. Add 22.5 ml of the 10 M NaOH solution prepared in step 1.2.1 to the graduated cylinder and fill to the 450 ml line with ultrapure water.
    4. Store the solutions in 500 ml amber glass bottles and label each solution with the date (use letters to distinguish each bottle).
    5. Once the solution is made, take a laboratory blank. Remove 25 ml of the solution and record from which solution bottle it came. Store blanks in 60 ml amber glass bottles.
      NOTE: Each solution bottle should yield 8-11 samples (35-50 ml each) and one field blank (25 ml) after the laboratory blank is taken.

2. Field Setup

  1. Choose a sampling location (such as a rooftop) and install the system (if using the stationary system). For the mobile laboratory, pack all instrumentation into a typical passenger vehicle. See Figure 1 for a diagram of the automated system.
  2. Change all filters labeled in Figure 1 before sampling to ensure that they are working most effectively and efficiently.
    NOTE: There are three types of filters in the system: a PTFE particle filter (1.0 µm; 47 or 25 mm use the larger surface area in more polluted air) to remove particles that may contain NO3-, a nylon membrane filter (1.0 µm) for removal of HNO3 gas, and a hydrophobic filter (10.0 µm) to protect the vacuum pump and the critical orifice from solution droplets. With new filters at the start of a sampling period, the particulate filter and the NO3 filter will not need to be changed for a couple of days, except in highly polluted or dusty conditions. The hydrophobic filter should be changed every 4-6 hr for as long as sampling is continuously done.
  3. For installation of the system, connect the system and the instruments to polytetrafluoroethylene (PTFE) tubing (1/4-inch outer diameter) and aim the inlet, also PTFE tubing, in the direction of the desired air collection.
    NOTE: The mobile laboratory is for taking on-road measurements, whereas the stationary laboratory is for taking ambient urban air and near-road measurements.
  4. Set up the 'mobile laboratory', consisting of the NOx collection system, an NOx box, a CO2 analyzer, a global positioning system (GPS) unit, and a marine battery.
    1. Pack the system and all instrumentation into the car. Power the system with a 12 V marine deep-cycle battery for ~12 hr, similar to the maximum duration of one day of mobile measurements. Recharge the battery at the end of the sampling day to prepare for the next day.
      NOTE: Use a separate battery so that there is no need to hardwire to the car battery nor to keep the car running to make measurements. Use two batteries if sampling will be close to or more than 12 hr in order to avoid stopping for a few hours to recharge the battery.
    2. Connect the instruments to the PTFE inlet tube closer to the inlet than to the collection system, because the vacuum pump for the system operates at flow rates of 3-5 L/min, much larger than the flow rates for the NOx box (~1.5 L/min) or the CO2/H2O analyzer (<1 L/min).
    3. Secure the PTFE inlet tube to the front of the car on the roof, pointing towards the front of the car, a position that is the longest possible distance from the exhaust pipe, to avoid capturing self-emissions from the car exhaust pipe. For example, in the mobile laboratory using a midsized sport utility vehicle, the inlet was located on the roof of the car, 2 feet from the driver's side door, placing it 1.6 meters above the road and 2.54 meters from the back bumper.
      1. Alternatively, use an electric or other zero emission vehicle.
    4. Record geolocation and vehicle speed data every second using a GPS unit (if this data is of interest). Synchronize the laptop computer recording the NOx and CO2 data with the GPS time prior to measurements.
    5. Turn on the instrumentation at the beginning of the sampling day and turn them off at the end of the sampling day, even when the collection system is not running (the instruments require warm-up time, so leave it running throughout the day to avoid multiple warm-up times).
    6. Load the NOx box with luminol solution when it is turned on at the beginning of the sampling day, and then flush it with water at the end of the day, before the instrument is turned off, as directed by the manufacturer. Store the luminol and solution samples in a cooler in the mobile laboratory to avoid degradation of the solutions. Store the luminol solution in a refrigerated unit overnight.
    7. Calibrate the NOx box-a luminol-based NO2/NO analyzer11 and a differential, non-dispersive infrared (NDIR) CO2/H2O analyzer using a commercial gas dilution calibrator and following the manufacturer's instructions. The NOx box has a response time of ~5 sec, which is better equipped to resolve on-road NOx emission plumes.
  5. Set up the 'stationary laboratory', consisting of the NOx collection system and a chemiluminescence NOx concentration analyzer.
    1. Fix PTFE tubing to a surface and point it in the direction of the air to be collected.
    2. Split the PTFE tubing at the inlet with a tee fitting to connect both the NOx analyzer and the automated collection system.
    3. Connect the stationary system to a power outlet (120 V alternating current).
    4. Run the NOx concentration analyzer continuously throughout the sampling period, even when the collection system is off or switching samples. Use the valve built into the collection system to isolate it during that time so that the NOx analyzer is sampling ambient air.
    5. Calibrate the chemiluminescence NOx concentration analyzer. This is used for the stationary measurements, as it has a slower response time (>30 sec), which is better for ambient air measurements.
      1. Calibrate based on the manufacturer's instructions using a gas dilution calibrator. Dilute a standard of 25 ppmv NO in N2 with zero air to achieve approximately seven calibration points between 0-200 ppbv NO. Using an ozone titrator, calibrate the NO2 concentrations across the same range (0-200 ppbv NO2).
    6. If using the mobile laboratory, calibrate the NOx box-a luminol-based NO2/NO analyzer11 and a differential, non-dispersive infrared (NDIR) CO2/H2O analyzer using a commercial gas dilution calibrator and following the manufacturer's instructions. The NOx box has a response time of ~5 sec, which is better equipped to resolve on-road NOx emission plumes.

3. Sample Collection

  1. Perform tests on the system to ensure that the flow meter, the syringe pump, the computer software, and the vacuum pump are all working. Turn on each component and check that it is working properly. With the computer software, complete the sampling protocol once or twice to make sure that everything is working properly.
  2. Turn on the system so that air is bubbling through the solution and bubbles are visible.
    NOTE: The computer program automates the movement of the solutions throughout the system, but the diaphragm pump and the vacuum pump are operated manually. The user must select the amount of time for the collection of a sample (between 30 and 120 min) that collects enough NOx for the concentration of the sample to be above the blank nitrate concentration of the solution. Atmospheric NOx concentrations of 50-100 ppbv near sources such as vehicles require only a 30 min collection time. For ambient urban NOx concentrations (5-30 ppbv), samples should be collected for up to 120 min. Using the equations provided in steps 6.5 and 6.5.1, the user can back-calculate the collection time to achieve the desired sample concentration in solution.
    1. Use a collection system with a syringe pump to automatically move the solution from the reservoirs into the gas-washing bottle and from the gas-washing bottle to the waste. The four electronically actuated valves and the syringe pump are controlled by a computer program specifically written for the collection system, which has four modes: 1) dispense new solution, 2) clean the tubing, 3) collect the sample, and 4) clean the gas-washing bottle, as follows:
    2. To automatically dispense new solution, aspirate 35 ml of solution (VS) into the syringe pump from the solution reservoir and dispense it into the gas-washing bottle. The gas-washing bottle frit causes the solution to bubble when the vacuum pump is turned on and the gas sample is introduced.
      NOTE: Choose a solution volume between 25-35 ml based on the NOx concentrations sampled and the collection times desired.
    3. Clean the tubing between the syringe pump and the gas-washing bottle by automatically pulling the residual solution in the tube back into the syringe and depositing it into the waste container.
    4. Once the sampling solution is in the gas-washing bottle, manually turn on the pump. When the desired amount of time for sampling has been achieved, manually turn off the pump.
    5. After sampling is done, collect the solution by opening the automated valve under the gas-washing bottle to drain the solution into a collection vial and cap via gravity. When the sample is done collecting NOx, collect the solution in a 60 ml amber glass bottle and manually remove the bottle. The program waits ~2 min for the solution to fully drain and then automatically moves on to the next step.
    6. Once the sample is done draining, automatically close the valve and clean the gas-washing bottle by aspirating ultrapure water into the syringe pump and dispensing it into the gas-washing bottle via a spray nozzle to clean the sides of the gas-washing bottle. Extract this wastewater from the gas-washing bottle by aspirating it into the syringe pump and discard it into the waste reservoir. Store the frit with 25 ml of nanopure water.
    7. Repeat steps 3.2.2 to 3.2.6 to dispense the next solution sample.
  3. Take field blanks during the collection for each solution bottle (labeled with the letters A-Z before the beginning of collection) that is used by sending 25 ml of solution through the system without turning on the vacuum pump to collect air. Collect the solution immediately after it is put into the system.
  4. Record the volumetric flow rate every 5 min using a flow meter during each collection, along with the air temperature (T) and pressure (P) on the flow meter, to derive the standard flow rate. Flow rates of 3-5 L/min are achieved with a diaphragm pump (30 L/min capacity) and a critical orifice to reduce the flow rate.
    1. Set the flow rate at the beginning of sampling to measure the flow approximately every 1 sec. After 5-10 sec, change the flow measurement frequency to 5 min.
    2. Collect the flow-rate data every 5 min for the duration of the sampling period.
      NOTE: If the flow rate drops significantly, the sample should be collected for longer than initially expected. Small changes (<25%) in the initial flow rate are to be expected. The hydrophobic filter should be checked to see if it is coated to the point that it is clogged.
    3. Before the sampling is stopped, change back to 1 sec flow-rate measurements and collect flow data for 5-10 sec before turning off the sample.
      NOTE: The solutions may be stored for up to (maximum) seven days before step 4 must be performed.

4. Sample Reduction

NOTE: Reduce the samples to remove the KMnO4 within 7 days of collection. The original method4 suggests that this must be done within 24 hr of sample collection. Below are results that suggest that the samples can be stored for up to seven days prior to reduction.

  1. The permanganate in solution is a strong oxidizer. Reduce the sample to stop the oxidation reaction of permanganate with ambient NOx or with other N species that could potentially lead to interference if they are oxidized to nitrate4.
  2. Label two 400 ml beakers, one for blank solutions and one for samples. Acquire two stir rods, one for each beaker. Also, acquire a 500 to 5,000 µl pipette and pipette tips.
  3. Weigh each sample bottle while it contains the solution and record the mass of the full glass bottle. After the solution has been poured into the beaker, weigh the empty glass bottle.
  4. Pour the solution from one sample into the sample beaker and one blank solution into the blank beaker.
  5. For the sample beaker, slowly introduce 10 ml of hydrogen peroxide (H2O2) in 5 ml batches to the sample, stirring vigorously while adding each 5 ml of H2O2. This volume is for sample solutions of 35 ml. For each 25 ml of sampling solution (either blank or sample), add 5 ml of H2O2. Adding the full 5 ml for less than 25 ml of sample solution is recommended to ensure full conversion, and adding more H2O2 will only result in the dilution of the solution.
    1. Introduce the first 5 ml of H2O2 above the beaker, so that the tip does not touch the beaker, the stir rod, or the solution.
    2. Add the second batch to the side of the beaker and around the side, as more is added, in order to wipe down the sides of the beaker, ensuring that all of the sample solution is reduced. If more than 5 ml of H2O2 is added, perform this step on the last 5 ml introduction of H2O2 and follow step 4.5.1 for the intermediary introductions of H2O2.
    3. Change the pipette tip after each sample is changed to avoid any cross-contamination.
  6. For the blank solution beaker, add only 5 ml of H2O2. Add roughly half of the above the solution and add the other half around the sides of the beaker. Change the tip after each blank.
  7. Check to ensure that the solution above the precipitate is clear or pale yellow. If purple or blue colors remain, add more H2O2 to verify that it is fully reduced.
  8. Pour the entire contents of the beaker, both the liquid and the brown precipitate that forms into 50-ml centrifuge tubes that have been labeled according to the sample or blank number or letter.
  9. Once all solutions are reduced (the H2O2 is added), load the centrifuge in batches of 20, ensuring that the centrifuge is balanced. A benchtop centrifuge can typically accommodate 20 centrifuge tubes at a time.
    1. Operate the centrifuge at 3,220 x g for 15 min with each batch of centrifuge tubes.
  10. While the centrifuge is running, weigh the empty glass bottles and record their masses. Additionally, label 60 ml amber plastic bottles (previously cleaned by leaching in ultrapure water) and weigh the empty plastic bottles. Record their masses as well.
  11. Once the centrifugation has finished, pour the supernatant liquid into the amber plastic bottle (with the solid remaining in the tube) and properly dispose of the centrifuge tube.
  12. Weigh the sample bottles, now full, and record their masses.

5. Sample Neutralization

NOTE: Perform the neutralization of samples and blanks (reproduced here from Fibiger et al.4 with updates). Note that this step is required for the colorimetric quantification of the nitrate concentration in the solution; this may not be necessary with other concentration techniques.

  1. Perform neutralization manually or with an automatic titrator.
  2. For manual neutralization, use 12.1 M hydrochloric acid (HCl) and introduce it into the amber plastic solution bottle with a pipette. Take extreme care (eyewear, lab coat, fume hood, etc.) when handling HCl, particularly at a 12.1 M concentration.
    1. Calculate the volume of 12.1 M HCl that is to be added to the sample solution to neutralize it using the following equation:
      Equation 1
      where VHCl is the volume of HCl added, massfull glass bottle is the mass of the glass bottle the solution was collected in with the solution, and massempty glass bottle is the mass of the glass bottle the solution was collected in without the solution. Assume that the densities of the solutions to be 1.00 g/cm3, as they are dilute solutions.
      NOTE: Add 85% of this volume first in 0.20 ml increments. Depending on the air that is being sampled, other species that are collected can lower the original solution pH and change how it responds to the addition of acid.
    2. Add the 85% volume of HCl to the bottle using a pipette and a disposable tip in 0.2 ml increments. Cap and shake the bottle between each 0.2 ml addition to ensure the acid has mixed with the solution. Check the pH using litmus paper by removing 20 µl of solution and pipetting it onto the litmus paper.
    3. If the pH is between 4 and 10, label the sample as neutralized and record the pH. Repeat steps 4.2.1 and 4.2.2 for all other samples being processed. While the colorimetric concentration analysis can proceed with samples that have a pH as low as 4 or as high as 10, get as close to 7 as possible in order to yield the best results.
    4. If the pH is still above 10 after the 85% volume addition, add HCl in smaller increments than 0.2 ml of HCl (0.10 or 0.05 ml), shake the bottle to homogenize, and check the pH with litmus paper and 20 µl of solution after each addition of HCl.
    5. Once the pH is within the desired range, label the sample as neutralized in the same way as before and set it aside.
    6. If the pH is below 4, use 10 M NaOH to bring the pH up to the correct range. Add increasingly small amounts of NaOH and check the pH after each addition using litmus paper and the same method as before.
    7. Record the amount of HCl (VHCl) and NaOH (if needed) added to each sample bottle, along with the final pH.
  3. For the automatic titrator method, use an automatic titrator.
    1. Dilute 12.1 M HCl to 4 M with ultrapure water and introduce it to the titrator (0-25 ml of hydrochloric acid are possible) according to the instructions of the instrument. 4 M allows the titrator to be precise enough without adding large volumes to the samples.
    2. Set the automatic titrator to titrate to a pH of 7.
    3. Record the final pH and the volume of HCl added. Label the sample as neutralized and record the final pH.
    4. Use the same beaker for each titration. In between each sample, wash the beaker, pH probe, and stirrer with ultrapure water at least 3 times and dry.

6. Sample Measurement

  1. Measure the concentration of each of the samples (CB or CS) using a spectrophotometric nutrient analyzer that utilizes colorimetric chemistry to make concentration measurements.
  2. Prepare the samples and put them into the instrument according to the manufacturer's instructions.
  3. Generate a standard calibration curve from 0-15 µM nitrate (7 calibration points) from a 30 µM stock KNO3 solution.
  4. Prepare and run quality controls of 8 and 10 µM nitrate along with the samples. Calculate the pooled standard deviation of QCs across multiple runs to estimate the concentration uncertainty. Typical pooled standard deviations of ±0.4 µM are observed (across 7 runs, N = 27 data points).
    1. Convert the NOx concentration determined by the colorimetric concentration analyzer from μM to ppbv using the following mixing ratio equation:
      Equation 2
      where MRNOx is the mixing ratio of NOx (the value reported in ppbv), nNOx is the number of nmol of NOx collected, R is the ideal gas constant in Equation 3, T is the temperature (in Kelvin), P is atmospheric pressure (in atm), and V is the volume of air (in L) collected. The total volume of gas sampled is determined by numerical integration of the flow rate time series (equivalent to the area under the flow rate curve as a function of time).
    2. Calculate the number of moles of NOx using the following set of equations:
      Equation 4
      for the sample,
      Equation 5
      for the blank, and
      Equation 6
      where CS is the concentration of the sample measured by the colorimetric concentration analyzer, in µmol; VS is the volume of the sample, in ml; CB is the concentration of the blank, in µmol; VB is the volume of the blank, in ml; Valiquot is the volume of what was neutralized, in ml (typically the entire volume of the solution); and VHCl is the volume of HCl added to neutralize the aliquot, in ml.

7. Nitrogen Isotope Ratio Preparation

NOTE: Quantify the nitrogen isotopic composition based upon the denitrifier method. Details of this method are published elsewhere in their entirety, and users should consult these publications for full method instructions12,13. The method utilizes denitrifying bacteria to convert liquid NO3- samples into gaseous nitrous oxide (N2O) for isotopic determination. Users who do not have the denitrifier method readily set up may have samples analyzed for isotopic composition by external facilities. Users should consult these facilities to ensure that the appropriate data corrections are consistent with those in step 8.

  1. Based upon the concentration determined for each sample and blank, inject the appropriate volumes into pre-prepared, capped vials with bacteria12,13. Target a specific size for injection by dividing the target size, such as 20 nmol N, by the concentration (µmol/L) of the sample or blank to determine the number of ml to inject into each vial via a syringe.
    1. Inject nitrate reference materials (e.g., IAEA-NO-3 and USGS34) at least in triplicate with each set of samples to be run for isotopes. These reference materials are used to correct the final data to standardized, internationally accepted values14.
    2. Fill two beakers with ultrapure water for rinsing the syringes between the injections of samples and reference materials. Obtain an empty bottle for waste solution.
    3. Dip the tip of the syringe being used for injection in the first beaker of water and dry it off. Rinse the full volume of the syringe with ultrapure water and discard the water as waste. Repeat three times.
    4. Following a similar procedure to step 3.2.3, fill the syringe with a small amount of sample to pre-rinse the syringe. Discard it as waste. Refill the syringe with sample and gently knock to remove any air bubbles so that an accurate volume is measured.
    5. Inject the sample into the sample vial.
      1. If there is more than 3 ml being injected into the sample, use a second "vent" needle to relieve pressure in the vial. Push the syringe with the sample into the rubber septa and begin injecting the sample. After 0.5 ml of the sample has been injected, insert the second "vent" needle. Leave the "vent" needle in until 1 ml is left to be injected, and then remove the "vent" needle. Continue injecting the last of the sample.
    6. Store the vials overnight in a warm (~24 °C) area. The following morning, inject 0.1 to 0.2 ml of 10 M NaOH into each sample to lyse the bacteria.

8. Isotope Ratio Determination

NOTE: Once the bacteria are lysed, the samples are ready to be run on the isotope ratio mass spectrometer (IRMS).

  1. Inject each sample with three to four drops of antifoam before setting them up to run on the mass spectrometer.
  2. Determine the isotopic composition by ion ratio mass spectrometry. Use a mass spectrometer interfaced with a modified system for the automated extraction, purification (removal of CO2 and H2O), and isotopic analysis of N2O at m/z 44, 45, and 4612, 13.
  3. Run a blank with only media solution and no sample in the vial at the beginning of each run.
  4. Calibrate the raw isotope ratios from the mass spectrometer using reference materials (e.g., IAEA-NO-3 and USGS 34) treated in the same manner as the samples, based on the correction scheme in Kaiser et al. (2007)15. This puts them in a form that is usable for data comparisons with other laboratories in the N isotope biogeochemistry community.
  5. Due to concerns about the linearity of the mass spectrometer values, if the area of the injected sample is outside of ±10% of the target area, adjust the concentration using that percentage and re-inject the sample, following the above procedure.
    1. To finalize the sample data for δ15N of NOx (the delta notation is defined using the following equation for δ15N: ("delta 15-N"): δ15N = [(15N/14Nsample/15N/14Nstandard) - 1] x 1,000‰ and the standard used for the nitrogen samples is atmospheric N2 gas), correct the δ15N for the contribution of the nitrate blank that is found in the KMnO4 solution:
      Equation 7
      where δ15Ntotal, measured is the value determined for the sample from the mass spectrometer run, δ15Nblank, measured is the value determined for the blank, and the Sample and Blank Concentrations are the values determined from the colorimetric analyses. This equation removes the effect of the blank from the isotopic ratio, so that the isotopic ratio is now representative of the NOx collected in situ.
      NOTE: The isotopic composition and concentration of the blank solution nitrate is measured with every batch of solution used. This blank is different from any potential blank that might be encountered with the denitrifier method alone (which is also quantified with every run). Any blank associated with the denitrifier method is true for all samples and reference materials; however, the permanganate solution blank is only applicable to samples and therefore must be quantified and corrected for (by mass balance) separately.

Representative Results

In the original method development work by Fibiger et al., the NOx collection method was rigorously tested in the laboratory under a variety of conditions4. Here, the focus is on updates to the method and field applications under a variety of environmental conditions. Results are reported on (1) field collection efficiency, (2) sample solution stability in terms of time before sample reduction and sensitivity to high concentrations of ammonium (NH4+) in solution, and (3) reproducibility in the field. The versatility of the method is demonstrated in its application for ambient air, near-road, and on-road measurements.

The average concentrations collected in solution were compared with those from 1 min NOx concentrations from the chemiluminescence NOx analyzer over a two-day diurnal study in ambient urban air in Providence, RI. Figure 2 details the collection efficiency during a period when concentrations varied over a large range, from ~2.5-18 ppbv NOx. Figure 2A displays a direct comparison of median NOx concentrations from the NOx analyzer compared with concentrations calculated from the solution and flow measurements, indicating that, on average, solution concentrations are 92% of median in situ concentrations. This falls within the expected uncertainty range of ±10%, but the difference likely reflects varying concentrations during the collection periods (Figure 2B). Based upon examination of the percentiles of the distribution of the 1-min NOx concentration data, the solution-based NOx concentrations are within the distribution for every collection interval (Figure 2B).

It has been recommended to complete the reduction of the samples collected in the field within 1 day after collection is complete (i.e., complete all of step 3). This target was suggested to reduce the potential for interference from the collection of other soluble nitrogen species, such as NH3, that could be converted to nitrate in the highly oxidizing KMnO4/NaOH solution over time. To test this more specifically, samples were collected in May and July 2015 in Providence, RI on the campus of Brown University, at a loading dock that is near a regularly traveled local road where diesel delivery trucks are regularly running in idling mode to unload. Samples were collected, and then aliquots of the samples were separated and reduced at different times (1 day, 4-7 days, and 13-15 days) after the sample collection (Figure 3A). Samples in Figure 3B were also collected during May and July but were prepared by adding 5 ml of 10 mM ammonium chloride to 450 ml of solution. This yielded a concentration of 111 µM NH4+ in solution, corresponding to collecting 220 ppbv of NH3 in the air, if only NH3 was collected. These concentrations are the maximum expected during on-road measurements near vehicle NH3 sources16. With or without the added NH4+, samples reduced within 7 days after collection had consistent isotope ratios when compared to the first reduction (within 1 day of collection), all falling within the expected uncertainty range of ±1.5% (Figure 3A and 3B). Note that the ±1.5% uncertainty is representative of isotopic determinations of repeated collections of tank NOx4. The uncertainty associated with repeated measures of isotopic reference materials alone is typically 0.3%. After two weeks, however, samples with or without added NH4+ were not necessarily stable. While in some cases the isotope values still appear to be consistent (e.g., Figure 3A), samples exhibited small NO3- concentration increases (<1 µM) when compared with the first reduction and, in some cases, decreases in NO3- concentrations. With the added NH4+, it would have been expected that NO3- concentration would increase over time above the expected uncertainty range (~0.8 µM) for concentration measurements, suggesting that even after two weeks, the NH4+ was not the source of interference. Further experiments are needed to better understand the source of this instability, though it is noted that blank solutions left untreated over the same time course consistently showed no change or slight increases in concentrations, and therefore, the instability must be created by the presence of other species found in the ambient urban air. Until this is resolved, it is recommended that sample solutions be reduced within 7 days from the time of collection.

Figure 4 details the collection of samples with the mobile setup over various field campaigns in urban, near-road, and on-road settings. The NOx concentration range spans three orders of magnitude, and the isotope ratios range from -1 to -13‰. This sampling set includes 51 on-road samples taken over 52 hr, covering over 4,000 km, and in a myriad of driving conditions (e.g., heavy stop-and-go traffic to very light traffic at high speeds on the highway). The sampling took place on roads in and between 6 major cities, including Providence, RI, Philadelphia and Pittsburgh, PA, and Cleveland, Columbus, and Cincinnati, OH. Average vehicle speeds ranged from 12.4 km/hr to 119.7 km/hr. The near-roadside samples (N = 27) were obtained at a monitoring site at I-95 in Providence, RI. The ambient urban air samples (N = 44 samples taken over 117.5 hr) were taken from two rooftop locations in Providence, RI, one near the I-95, I-195 interchange and one 775 meters away from the interchange site. This represents the first steps towards building new capabilities to resolve the ranges of isotopic signatures from NOx sources, in this case, vehicle emissions and ambient urban sources. The variations in daytime on-road and road-side δ15N-NOx (Figure 4) were not correlated with variations in driving conditions and occurred across relatively constant vehicle fuel-class traffic counts. A more detailed discussion of the variations of isotopic signatures due to vehicle fuel types is the subject of another manuscript (Miller, D.J., et al. 2016. J. Geophys. Atmos. Submitted).

Finally, Table 1 details field and laboratory collections where two collection systems were deployed at the same time to test reproducibility. The comparisons show excellent agreement for the isotopic data, quantified here as the absolute deviation between the two data points for each collection period. The data are displayed from urban air collections at a rooftop location in Providence, RI; near-roadside collections in Providence, RI; and from collections in a laboratory-based smog chamber at the University of Massachusetts, Amherst.

Figure 1
Figure 1: Collection Schematic and Image. (A) Diagram of the Automated NOx Collection System. Gray is airflow, blue is water/solution flow, green is electronics connections, yellow is the frit, and purple is the permanganate solution. The syringe pump is used to add and remove rinsing solution (ultrapure water) and to add new solution for the start of sample collection (the syringe pump is a commercially available stepper motor syringe pump with a 50 ml syringe, a 5-port distribution valve, and driver/control boards equipped with an RS-232 serial interface). The sample is removed manually via the black valve at the bottom of the gas washing bottle. (B) Picture of the NOx collection system and NOx box in the mobile laboratory. Please click here to view a larger version of this figure.

Figure 2
Figure 2: Collection Efficiency of the Automated Collection System. (A) The NOx concentrations calculated from the NO3- concentrations measured in solution and the flow data compared against the median concentration measured by a chemiluminescent NOx concentration analyzer at a rooftop site in Providence, RI. The error bars are the standard deviation (±1σ) of the solution-based NOx mixing ratio estimates derived from the propagated errors of the pooled standard deviations of the quality controls (0.4 µM) across colorimetric concentration measurement runs and the flow rate uncertainty (±1%). The NOx analyzer concentration uncertainties are ±5%. (B) The time series of NOx concentration distributions during diurnal measurements at a rooftop site in Providence, RI. The boxes represent the 25th, 50th, and 75th percentiles. The whiskers represent the extremes without outliers. Please click here to view a larger version of this figure.

Figure 3
Figure 3: Comparison of the reduction times for NOx samples collected at Brown University in May and July 2015. (A) The results are recorded as deviations from the first reduction, performed within 1 day of sampling. May samples are displayed as triangles and July samples as circles, with colors denoting different collection periods. Samples in (B) were pre-treated with ammonium chloride prior to air collection to test the interference of NH4+ in solution over time. The dashed lines represent the expected overall precision of the isotopic collection method, expressed as a standard deviation of ±1.5%. Please click here to view a larger version of this figure.

Figure 4
Figure 4: The δ15N-NOx () and NOx concentration of samples collected in ambient urban air, on-road, and near-road sites. The types of samples are delineated by different colors, and represent a range of conditions (see the text) and NOx concentrations. Please click here to view a larger version of this figure.


 

Sample Name System Number Collection Date Hours of Collection Temperature (ºC) [NO3-] (µM) Blank/total N δ 15N (‰) Deviation δ 15N (%)
Urban Air PVD 1 1 10/8/2013 - 10/9/2013 6.75 15.8 14.43 0.3 -0.6 0.7
2 16.78 0.26 -1.3
Urban Air PVD 2 1 11/6/2013 - 11/7/2013 2.5* 17.1 30.86 0.2 -7.7 1
2 5.25 37.05 0.17 -6.7
Urban Air PVD 3 1 11/20/2013 - 11/21/2013 8.9 3.28 44.29 0.14 -7.1 0.4
2 29.66 0.21 -6.7
Near Roadside 1 1 8/14/2014 - 8/15/2014 29 19.2 13.3 0.37 -9.47 0.69
2 16.4 0.3 -10.16
Near Roadside 2 1 8/17/2014 - 8/18/2014 30 21.85 9.4 0.68 -8.95 1.56
2 11.6 0.55 -7.39
Near Roadside 3 1 5/25/2015 3.5 20 6.86 0.51 -7.67 0.86
2 9.49 0.42 -8.53
Near Roadside 4 1 5/26/2015 2.75 25.56 6.07 0.656 -8.7 1.57
2 6.49 0.61 -7.13
Smog Chamber 1 1 8/26/2014 - 8/27/2014 24.4 21 24.392 0.27 -12.28 0.33
2 33.2 0.2 -12.61
Smog Chamber 2 1 8/27/2014 - 8/28/2014 19.8 21 10.96 0.54 -10.22 1.25
1 14.245 0.41 -11.47
Smog Chamber 3 1 8/28/2014 - 8/29/2014 24.2 21 7.476 0.8 -5.86 1.27

Table 1: Reproducibility of samples collected at the same time using two identical collection systems. *The collection had to be stopped due to a clogged filter. Urban Air PVD (PVD = Providence, RI) 1-3 were previously published4. Near Roadside represents roadside collections in Providence, RI; Smog chamber represents samples collected from air inside a smog chamber at the University of Massachusetts, Amherst4.

Discussion

The protocol above details the steps involved, from the field collection of air samples in solution to the laboratory processing of these solutions, to yield concentration and isotopic results. The critical steps in this protocol include comparing NOx analyzer measurements, minimizing the time before the reduction of solutions. and maintaining stable flow rates. If directly comparing solutions with in situ measurements of NOx concentrations, it is very important that an NOx analyzer is calibrated for ranges relevant for the chosen environment and that short-term variability in NOx concentrations be understood in the context of the longer time collections for the solutions. The accurate determination of solution NO3- concentrations is also important, both for calculation of the airborne NOx concentrations and for determining accurate injection volumes for the isotopic denitrifier method. The time period of solution stability before sample reduction is important to ensure consistent isotope ratios. As a result of the oxidizing potential of the solution, it is possible to oxidize in solution other reactive nitrogen species, most notably NH3, as it can be in high enough concentrations in certain areas to potentially affect the concentration of NO3- in solution. The oxidation of NH4+ to NO3- is expected to take longer than the oxidation of NOx to NO3-, so it had been recommended to reduce the samples (and thus stop the reaction) within 1 day of sample collection. Given that field conditions may result in the requirement of longer solution storage times, the stability of solutions was tested by examining solutions with and without added ammonium. With and without the addition of ammonium chloride, concentration and isotope values were stable within the 1σ uncertainty range (1.5‰) for up to one week (Figure 3). At two weeks after sampling, solutions with or without added NH4+ were not stable, in that NO3- concentration decreases were observed in some cases and blank corrections were no longer robust. Although it was expected that NO3- might increase over time due to NH4+ oxidation, decreases in concentration were actually observed in some cases, suggesting that even after two weeks, NH4+ interference is not causing the instability. As such, solutions should be reduced within one week, particularly if sampling is done in an environment with high NH3 concentrations (e.g., >200 ppbv). Finally, it is also critical to record the flow rate during field collections. The flow rate measured at the inlet was found to vary considerably and is difficult to control, even with a critical orifice in the system, since it can be influenced by the clogging of the hydrophobic filters and/or the frit. It is recommended to record the flow rate periodically (e.g., at 5 min intervals) throughout collections periods, such that the volume of air collected over time for each sample can be accurately determined (see step 5).

There are several alternatives or possible modifications of the protocols presented. For example, an important advantage of the denitrifier method is the low sample size requirement12,13. However, other isotopic methods may be used. Similarly, we use colorimetric determination of concentration, but other methods can yield accurate NO3- concentration results.

Collection efficiency in the field, as detailed in Figure 2, is 92 ± 10%. This is critical to ensure that there is no fractionation during the collection process. With collection efficiency less than 100%, fractionation in the collection process can occur, biasing the resulting isotopic ratios measured. The efficacy of this new collection method across a range of conditions in urban-influenced air has been shown. Table 1 outlines the multiple tests that were done under ambient-air, near-roadside, and smog-chamber sampling conditions to determine the reproducibility of the method. All isotope ratio differences between systems are <1.57‰. This demonstrates the reproducibility of this method over a range of different sampling conditions. The field-based method has a precision and reproducibility significantly better than the ~12‰ isotopic ratio variations observed in the environment (Figure 4).

The most significant limitation of the method is the NO3- blank or background associated with the KMnO4 solution. A variety of KMnO4 types have been tested (e.g., crystals, powders, and stock solutions)4, and all contained NO3- before being exposed to NOx in the air. As a result, it is necessary to collect enough NOx as NO3- in solution to achieve a concentration above the blank. Further studies are currently underway to quantify the level at which the sample should exceed the blank concentration for the most accurate results. Under very low ambient NOx concentrations, it may be necessary to modify the collection conditions to maximize the sample concentration. For instance, the flow rate could be increased to collect more air in a shorter timeframe or the solution volume could be reduced to increase the air-to-solution volume and to concentrate the air collection. In any case, the solution must remain above the frit in the collection vessel to maintain the bubbling of air through the solution.

This method of NOx collection for isotopic analysis is unique among existing methods (e.g., passive samplers6,17 and sulfuric acid and hydrogen peroxide solution8) in that it has been laboratory- and field-verified with respect to field applicability, reproducibility, sample solution stability, and efficiency of collection under a range of field conditions. This novel method is unique in its capabilities to actively collect NOx in field environments for isotopic analysis at ambient concentrations at a 30-120 min time resolution. It collects NOx at near 100% efficiency and has been demonstrated repeatedly to be reproducible within the range of uncertainty of the method. Sample solutions collected in the field remain stable for up to 1 week before needing to be reduced. The method can collect samples over a range of concentrations and isotope ratios, and it is shown to be reproducible from collection to collection. This technique can be used for sampling under a variety of different conditions, including on-road, using the mobile laboratory approach outlined in the protocol. The interpretation of the spatiotemporal variability in vehicular emissions of NOx is the subject of a separate manuscript, in preparation (Miller, D.J., et al. 2016.  J. Geophys. Atmos. Submitted).

Future sampling includes the application of this method to other types of NOx emissions (e.g., microbially produced emissions in soils and biomass fires). Isotopes are a potential way to track NOx sources, but only if different source signatures can be quantified and understood. Our new method makes it possible to quantify the isotopic composition of NOx from a variety of NOx emission sources and to directly test whether the impacts of emissions in the environment can be directly and quantitatively tracked.

Disclosures

The authors declare that they have no competing financial interests.

Acknowledgments

PKW would like to thank the Voss Environmental Fellowship at Brown University for funding and support. This research was funded by a National Science Foundation CAREER Award (AGS-1351932) to MGH. The authors thank Ruby Ho for her support of this project. The authors would also like to thank Rebecca Ryals; Jim Tang and his research group at the Marine Biological Laboratory, specifically Elizabeth de la Reguera, for access to the LMA-3d and Li-COR 7000 analyzers; Barbara Morin at the Rhode Island Department of Environmental Management; Roy Heaton and Paul Theroux at the Rhode Island Department of Health; Adam McGovern at the Warren Alpert Medical School; and Charlie Vickers and Tom Keifer for collection system machining. We also thank the editorial staff and two anonymous reviewers for their excellent suggested edits that improved the manuscript.

Materials

Name Company Catalog Number Comments
Gas Washing Bottle Custom Design used, numerous companies sell other gas washing bottles. The bottle needs to have a frit inside it.
Syringe Pump Kloehn Kloehn Versa Pump 6, 55 Series
PTFE Isolation Valves Parker 002-00170-900 Both 2 three way and double two-way normally closed, electronically actuated valves
Gas Handling Teflon Tubing McMaster Carr 5033K31 Quarter inch outer diameter, eigth inch inner diameter, PTFE tubing
Liquid Handling Tygon Tubing McMaster Carr 5103K32 Quarter inch outer diameter, eigth inch inner diameter, PTFE tubing
Compression gas fittings and ball valves (assorted) Swagelok Assorted Stainless Steel
Flow calibrator MesaLabs Defender 520
Compression PFA fittings Cole Parmer Assorted Gas and liquid handling
Data Acquisition Board National Instruments NI USB-6001 Used for valve switching
Solid State Relay Crydom DC60S5 Used for valve switching
Single Stage Filter Assembly Savillex 401-21-25-50-21-2 Use 25 mm and 47 mm diameter holders
Nylon Membrane Filter Pall Corporation 66509 1 μm filter, both 25 mm and 47 mm diameter filters
Hydrophobic Membrane Filter Millipore LCWP04700 10.0 μm, 25 mm and 47 mm diameter filters
Particle Membrane Filters Millipore FALP04700 1 μm filter, both 25 mm and 47 mm diameter filters
Mini Diaphragm Pump KNF UN 816.1.2 KTP Used for stationary lab
Mini Diaphragm Pump KNF PJ 26078-811 Used for mobile lab
Aluminum Onlinemetals.com 6061-T6 Cut to size to build system
Deep Cycle Power Battery EverStart 24DC
MilliQ Water Millipore ZMQSP0DE1
Potassium Permanganate 1 N Solution Fischer Scientific SP282-1
Sodium Hydroxide Pellets Fischer Scientific S318-1
Ohaus Benchtop scale Pioneer EX224N
4 ounce Amber Glass Bottles Qorpak Bottles GLC-01926 (60 ml and WM 125 ml bottles)
Amber HDPE Bottles Fischerbrand 300751 Part number given for 125 ml narrow mouth bottles, Two varieties (125 wide mouth and narrow mouth of some volume)
Pre-cleaned EPA Amber Wide-mouth Bottle, 500 ml Cole Parmer EW-99540-55
Hydrogen Peroxide 30% Fischer Chemical H325-500 Corrosive
Centrifuge 5810 R Eppendorf 5821020010
50 ml Polypropylene Conical Tube Falcon 14-432-22
12 N Hydrochloric acid Fischer Scientific A114SI212 Corrosive
Colorimetric Nutrient Analyzer Westco Scientific Instruments SmartChem 170 In purchasing the Colorimetric Nutrient Analyzer, this comes with buffers, cleaning solutions, rinse solutions, and solutions for running the instrument, including the solutions to be able to activate the cadmium column in the instrument for nitrate analysis.
Automatic Titrator Hanna Instruments HI 901
20 ml Clr Headspace Vial Microleter, a WHEATON Company W225283 Information listed  is for 20 ml vials. 50 ml vials can also be purchased from the vendor listed.
Septa, 20 mm Gray Butyl Stopper Microleter, a WHEATON Company 20-0025
Seal, 20 mm Standard Aluminium Microleter, a WHEATON Company 20-0000AS
25 G x 1 1/2 BD PrecisionGlide Needle BD 305127
26 G x 1/2 BD PrecisionGlide Needle BD 305111
Helium 05078-536 Can order from many different soures
Crimper/Uncrimper WHEATON 61010-1
Isopropanol Fischer Chemical A459-1
Syringes of varying size for mass spec injection BD Varies based on size
Antifoam B Emulsion Sigma-Aldrich A5757-500ML
IRMS ThermoFischer Scientific IQLAAMGAATFADEMBHW The actual isotope ratio mass spectrometer is listed here. Our set up also includes a gas bench and an autosampler.
Gass Bench II ThermoFischer Scientific IQLAAEGAATFAETMAGD
TriPlus RSH™ Autosampler ThermoFischer Scientific 1R77010-0200 Choose product for headspace injection
42i NOx Concentration Analyzer ThermoFischer Scientific 101350-00
NOx Box Drummond Technologies LMA-3D/LNC-3D
CO2 analyzer Licor 7000 7000
GPS Garmin 010-00321-31
Model 146i Dynamic Gas Calibrator ThermoFischer Scientific 102482-00
Model 111 Zero Air Supply ThermoFischer Scientific 7734
50.2 ppm NO in N2 Gas standard Praxis Air Will vary with each tank of standard air purchased

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References

  1. Galloway, J. N., et al. The Nitrogen Cascade. BioScience. 53, (4), 341-356 (2003).
  2. Levy, H. II, Moxim, W. J., Klonecki, A. A., Kasibhatla, P. S. Simulated tropospheric NOx: Its evaluation, global distribution and individual source contributions. J. Geophysical Research. 104, (D21), 26279-26306 (1999).
  3. Hastings, M. G., Casciotti, K. L., Elliott, E. M. Stable Isotopes as Tracers of Anthropogenic Nitrogen Sources, Deposition, and Impacts. Elements. 9, (5), 339-344 (2013).
  4. Fibiger, D. L., Hastings, M. G., Lew, A. F., Peltier, R. E. Collection of NO and NO2 for Isotopic Analysis of NOx Emissions. Anal. Chem. 86, (24), 12115-12121 (2014).
  5. Ammann, M., et al. Estimating the uptake of traffic-derived NO2 from 15N abundance in Norway spruce needles. Oecologia. 118, (2), 124-131 (1999).
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  8. Walters, W. W., Goodwin, S. R., Michalski, G. Nitrogen Stable Isotope Composition (δ15N) of Vehicle-Emitted NOx. Environ. Sci. Technol. 49, (4), 2278-2285 (2015).
  9. Zaveri, R. A., et al. Ozone production efficiency and NOx depletion in an urban plume: Interpretation of field observations and implications for evaluating O3-NOxVOC sensitivity. J. Geophys. Res. 108, (D14), 4436 (2003).
  10. Margeson, J. H., et al. An integrated method for determining nitrogen oxide (NOx) emissions at nitric acid plants. Anal. Chem. 56, (13), 2607-2610 (1984).
  11. Drummond, J. W., et al. New Technologies for Use in Acid Deposition Networks, ASTM STD. 1052. 133-149 (1990).
  12. Sigman, D. M., et al. A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater. Anal. Chem. 73, (17), 4145-4153 (2001).
  13. Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., Hilkert, A. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem. 74, (19), 4905-4912 (2002).
  14. Böhkle, J. K., Mroczkowski, S. J., Coplen, T. B. Oxygen isotopes in nitrate: new reference materials for 18O:17O:16O measurements and observations on nitrate-water equilibrium. Rapid Commun. Mass Spectrom. 17, (16), 1835-1846 (2003).
  15. Kaiser, J., Hastings, M. G., Houlton, B. Z., Röckmann, T., Sigman, D. M. Tripe oxygen isotope analysis of nitrate using the denitrifier method and thermal decomposition of N2O. Anal Chem. 79, (2), 599-607 (2007).
  16. Sun, K., Tao, L., Miller, D. J., Khan, M. A., Zondlo, M. A. On-Road Ammonia Emissions Characterized by Mobile, Open-Path Measurements. Environ. Sci. Technol. 48, (7), 3943-3950 (2014).
  17. Dahal, B., Hastings, M. G. Technical considerations for the use of passive samplers to quantify the isotopic composition of NOx and NO2 using the denitrifier method. Atmospheric Environment. (2016).

Erratum

Formal Correction: Erratum: Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
Posted by JoVE Editors on 01/04/2019. Citeable Link.

An erratum was issued for: Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx. An author name was updated.

One of the authors' names was corrected from:

Mary O'Conner

to:

Mary O'Connor

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