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JoVE Journal Genetics
Nachweis der microRNA-Expression in der peritonealen Membran von Ratten unter Verwendung der quantitativen Echtzeit-PCR
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Nachweis der microRNA-Expression in der peritonealen Membran von Ratten unter Verwendung der quantitativen Echtzeit-PCR

Article DOI: 10.3791/55505-v • 08:56 min • June 27th, 2017
June 27th, 2017 •
1, 2, 2, 2, 2, 2, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Hier stellen wir ein Protokoll für den Nachweis der microRNA-Expression in der Ratten-Peritonealmembran unter Verwendung einer quantitativen Echtzeit-Reverse-Transkription-Polymerase-Kettenreaktion vor. Diese Methode eignet sich zur Untersuchung des microRNA-Expressionsprofils in der Ratten-Peritonealmembran in mehreren pathologischen Zuständen.

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Molekularbiologie Ausgabe 124 MicroRNA Ratte Peritonealmembran Fibrose Reinigung Nachweis qRT-PCR
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