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JoVE Journal Genetics
Optimering og sammenligningsanalyse af planteorganell DNA-berigelsesmetoder egnet til næste generations sekventering
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Optimering og sammenligningsanalyse af planteorganell DNA-berigelsesmetoder egnet til næste generations sekventering

Article DOI: 10.3791/55528-v • 12:33 min • July 28th, 2017
July 28th, 2017 •
*1,2, *1,3, 1,3
1Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science, University of Minnesota, 3Department of Plant Pathology, University of Minnesota
* These authors contributed equally

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Sammenligningen og optimeringen af ​​to planteorganelle DNA-berigelsesmetoder er præsenteret: traditionel differentiel centrifugering og fraktionering af det totale gDNA baseret på methyleringsstatus. Vi vurderer den resulterende DNA-mængde og kvalitet, demonstrerer ydeevne ved kortlæsning af næste generations sekventering og diskuterer potentialet til brug i langlæsende enkeltmolekylære sekventering.

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Genetik udgave 125 chloroplast genomik mitokondrier næste generations sekventering organelle DNA isolering planter hvede
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